Manipulation of ZDS in tomato exposes carotenoid- and ABA-specific effects on fruit development and ripening.

Autor: McQuinn RP; Department of Plant Biology, Cornell University, Ithaca, NY, USA.; Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, NY, USA., Gapper NE; Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, NY, USA., Gray AG; Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, NY, USA., Zhong S; Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, NY, USA., Tohge T; Max-Planck-Institut fur Molekulare Pflanzenphysiologie, Potsdam-Golm, Germany., Fei Z; Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, NY, USA., Fernie AR; Max-Planck-Institut fur Molekulare Pflanzenphysiologie, Potsdam-Golm, Germany., Giovannoni JJ; Department of Plant Biology, Cornell University, Ithaca, NY, USA.; Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, NY, USA.; Robert W. Holley Center for Agriculture and Health, USDA-ARS, Cornell University, Ithaca, NY, USA.
Jazyk: angličtina
Zdroj: Plant biotechnology journal [Plant Biotechnol J] 2020 Nov; Vol. 18 (11), pp. 2210-2224. Date of Electronic Publication: 2020 Apr 20.
DOI: 10.1111/pbi.13377
Abstrakt: Spontaneous mutations in fruit-specific carotenoid biosynthetic genes of tomato (Solanum lycopersicum) have led to improved understanding of ripening-associated carotenogenesis. Here, we confirm that ZDS is encoded by a single gene in tomato transcriptionally regulated by ripening transcription factors RIN, NOR and ethylene. Manipulation of ZDS was achieved through transgenic repression and heterologous over-expression in tomato. CaMV 35S-driven RNAi repression inhibited carotenoid biosynthesis in all aerial tissues examined resulting in elevated levels of ζ-carotene isomers and upstream carotenoids, while downstream all trans-lycopene and subsequent photoprotective carotenes and xanthophylls were diminished. Consequently, immature fruit displayed photo-bleaching consistent with reduced levels of the photoprotective carotenes and developmental phenotypes related to a reduction in the carotenoid-derived phytohormone abscisic acid (ABA). ZDS-repressed ripe fruit was devoid of the characteristic red carotenoid, all trans-lycopene and displayed brilliant yellow pigmentation due to elevated 9,9' di-cis-ζ-carotene. Over-expression of the Arabidopsis thaliana ZDS (AtZDS) gene bypassed endogenous co-suppression and revealed ZDS as an additional bottleneck in ripening-associated carotenogenesis of tomato. Quantitation of carotenoids in addition to multiple ripening parameters in ZDS-altered lines and ABA-deficient fruit-specific carotenoid mutants was used to separate phenotypic consequences of ABA from other effects of ZDS manipulation and reveal a unique and dynamic ζ-carotene isomer profile in ripe fruit.
(© 2020 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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