Establishment of Murine Model of Kidney Failure Induced by Severe Ischemia-Reperfusion Injury Useful to Evaluate Transplantation and Regenerative Therapies.

Autor: Najar-Acosta LJ; Laboratory of Tissue Engineering and Transplant, Department of Physiology, University Center for Health Sciences, University of Guadalajara, Jalisco, Mexico; cGMP Cell Processing Facility, University Center for Health Sciences, University of Guadalajara, Mexico., Robles-Murillo AK; Laboratory of Tissue Engineering and Transplant, Department of Physiology, University Center for Health Sciences, University of Guadalajara, Jalisco, Mexico; cGMP Cell Processing Facility, University Center for Health Sciences, University of Guadalajara, Mexico., León-Moreno LC; Laboratory of Tissue Engineering and Transplant, Department of Physiology, University Center for Health Sciences, University of Guadalajara, Jalisco, Mexico., Desentis-Desentis MF; Laboratory of Tissue Engineering and Transplant, Department of Physiology, University Center for Health Sciences, University of Guadalajara, Jalisco, Mexico., García-Espinoza JA; Laboratory of Tissue Engineering and Transplant, Department of Physiology, University Center for Health Sciences, University of Guadalajara, Jalisco, Mexico., Barba-Gutiérrez JP; Laboratory of Tissue Engineering and Transplant, Department of Physiology, University Center for Health Sciences, University of Guadalajara, Jalisco, Mexico., Romero-Gómez AG; Laboratory of Tissue Engineering and Transplant, Department of Physiology, University Center for Health Sciences, University of Guadalajara, Jalisco, Mexico., Del Toro-Arreola A; Biobank of Genetic Material, Cells and Tissues for Biomedical Research, University Center for Health Sciences, University of Guadalajara, Jalisco, Mexico., Daneri-Navarro A; Biobank of Genetic Material, Cells and Tissues for Biomedical Research, University Center for Health Sciences, University of Guadalajara, Jalisco, Mexico., Topete-Camacho A; Biobank of Genetic Material, Cells and Tissues for Biomedical Research, University Center for Health Sciences, University of Guadalajara, Jalisco, Mexico., Franco-Topete RA; Department of Microbiology and Pathology, University Center for Health Sciences, University of Guadalajara, Jalisco, Mexico., Sánchez-Zubieta FA; cGMP Cell Processing Facility, University Center for Health Sciences, University of Guadalajara, Mexico; Department of Human Reproduction Clinics, University Center for Health Sciences, University of Guadalajara, Jalisco, Mexico., Zepeda-Moreno A; cGMP Cell Processing Facility, University Center for Health Sciences, University of Guadalajara, Mexico; Department of Human Reproduction Clinics, University Center for Health Sciences, University of Guadalajara, Jalisco, Mexico., Rivas-Carrillo JD; Laboratory of Tissue Engineering and Transplant, Department of Physiology, University Center for Health Sciences, University of Guadalajara, Jalisco, Mexico; cGMP Cell Processing Facility, University Center for Health Sciences, University of Guadalajara, Mexico. Electronic address: jorgerivas@inicia.edu.mx.
Jazyk: angličtina
Zdroj: Transplantation proceedings [Transplant Proc] 2020 May; Vol. 52 (4), pp. 1202-1205. Date of Electronic Publication: 2020 Mar 10.
DOI: 10.1016/j.transproceed.2020.02.014
Abstrakt: Background: Severe ischemia-reperfusion injury (SIRI) seems to be the key factor that can significantly affect the function of both native kidneys and renal allografts. Therefore, the development of a successful strategy is of a paramount importance in both basic and clinical research.
Methods: To determine the effects of SIRI on the native kidney function, a murine model was planned as follows: group 1 (n = 6) mice underwent to nephrectomy plus ischemia-reperfusion injury for 30 minutes; group 2 (n = 6) mice underwent to nephrectomy without ischemia-reperfusion injury and thus served as sham controls for SIRI. The results of serum creatinine (SCr) were analyzed using Mann-Whitney U tests to calculate the significance between mean values. Survival between groups was measured by Kaplan-Meier test.
Results: To reliably achieve an elevation of SCr levels animals were exposed to a SIRI. The values of SCr increased from 0.35 (SD, 0.09) mg/dL to about 2-fold within 2 days and 3-fold within the following 5 days. Under these given conditions the mice displayed signs and histologic findings of severe kidney damage. The survival rate was about 83% of the animals within a week, and they showed no capacity of complete spontaneous self-regeneration.
Conclusions: In this study, we aim to establish a murine model with extensive structural kidney damage and significant elevation of SCr levels, which could be used in basic and translational research of transplantation and regenerative therapies.
(Copyright © 2020 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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