The cyanobacterial saxitoxin exacerbates neural cell death and brain malformations induced by Zika virus.

Autor: Pedrosa CDSG; D'Or Institute for Research and Education (IDOR), Rio de Janeiro, Rio de Janeiro, Brazil., Souza LRQ; D'Or Institute for Research and Education (IDOR), Rio de Janeiro, Rio de Janeiro, Brazil., Gomes TA; Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil.; Laboratory of Cellular Microbiology, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Rio de Janeiro, Brazil., de Lima CVF; D'Or Institute for Research and Education (IDOR), Rio de Janeiro, Rio de Janeiro, Brazil., Ledur PF; D'Or Institute for Research and Education (IDOR), Rio de Janeiro, Rio de Janeiro, Brazil., Karmirian K; D'Or Institute for Research and Education (IDOR), Rio de Janeiro, Rio de Janeiro, Brazil.; Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil., Barbeito-Andres J; Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil., Costa MDN; Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil., Higa LM; Department of Genetics, Institute of Biology, Federal University of Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil., Rossi ÁD; Department of Genetics, Institute of Biology, Federal University of Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil., Bellio M; Institute of Microbiology Paulo de Goes, Federal University of Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil., Tanuri A; Department of Genetics, Institute of Biology, Federal University of Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil., Prata-Barbosa A; D'Or Institute for Research and Education (IDOR), Rio de Janeiro, Rio de Janeiro, Brazil., Tovar-Moll F; D'Or Institute for Research and Education (IDOR), Rio de Janeiro, Rio de Janeiro, Brazil.; Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil., Garcez PP; Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil., Lara FA; Laboratory of Cellular Microbiology, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Rio de Janeiro, Brazil., Molica RJR; Academic Unit of Garanhuns, Federal Rural University of Pernambuco, Garanhuns, Pernambuco, Brazil., Rehen SK; D'Or Institute for Research and Education (IDOR), Rio de Janeiro, Rio de Janeiro, Brazil.; Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil.
Jazyk: angličtina
Zdroj: PLoS neglected tropical diseases [PLoS Negl Trop Dis] 2020 Mar 12; Vol. 14 (3), pp. e0008060. Date of Electronic Publication: 2020 Mar 12 (Print Publication: 2020).
DOI: 10.1371/journal.pntd.0008060
Abstrakt: The northeast (NE) region of Brazil commonly goes through drought periods, which favor cyanobacterial blooms, capable of producing neurotoxins with implications for human and animal health. The most severe dry spell in the history of Brazil occurred between 2012 and 2016. Coincidently, the highest incidence of microcephaly associated with the Zika virus (ZIKV) outbreak took place in the NE region of Brazil during the same years. In this work, we tested the hypothesis that saxitoxin (STX), a neurotoxin produced in South America by the freshwater cyanobacteria Raphidiopsis raciborskii, could have contributed to the most severe Congenital Zika Syndrome (CZS) profile described worldwide. Quality surveillance showed higher cyanobacteria amounts and STX occurrence in human drinking water supplies of NE compared to other regions of Brazil. Experimentally, we described that STX doubled the quantity of ZIKV-induced neural cell death in progenitor areas of human brain organoids, while the chronic ingestion of water contaminated with STX before and during gestation caused brain abnormalities in offspring of ZIKV-infected immunocompetent C57BL/6J mice. Our data indicate that saxitoxin-producing cyanobacteria is overspread in water reservoirs of the NE and might have acted as a co-insult to ZIKV infection in Brazil. These results raise a public health concern regarding the consequences of arbovirus outbreaks happening in areas with droughts and/or frequent freshwater cyanobacterial blooms.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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