CAMPANELLIProgrammed Death 1 (PD-1) and PD-1 Ligand (PD-L1) Expression in Chronic Apical Periodontitis.

Autor:	Delgado RJR; Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, Brazil., Pinheiro CR; Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, Brazil., Gasparoto TH; Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, Brazil., Sipert CR; Department of Restorative Dentistry, Discipline of Endodontics, Dental School, University of São Paulo, São Paulo, SP, Brazil., de Moraes IG; Department of Operative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo, Bauru, Brazil., Garcia RB; Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, Brazil., Bramante CM; Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, Brazil., Bernardineli N; Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, Brazil., Nishiyama CK; Department of Endodontics Division, Scientific Coordinator of Endodontics Specialty, Dentistry Postgraduate Center, CPO Uningá, Bauru, Sao Paulo, Brazil., da Silva JS; Department of Biochemistry and Immunology, School of Medicine of Ribeirão Preto-University of São Paulo, Ribeirão Preto, SP, Brazil., Torres SA; Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, Brazil., Garlet GP; Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, Brazil., Campanelli AP; Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, Brazil.
Jazyk:	angličtina
Zdroj:	European endodontic journal [Eur Endod J] 2019 Feb 05; Vol. 4 (1), pp. 3-8. Date of Electronic Publication: 2019 Feb 05 (Print Publication: 2019).
DOI:	10.14744/eej.2018.46330
Abstrakt:	Objective: This study aimed to examine programmed death protein 1 (PD-1) and programmed death lig-and 1 (PD-L1) expression on leukocytes from chronic apical periodontitis, and to determine the levels of cytokines in the apical periodontitis lesions. Methods: Leukocytes from healthy gingival tissue (n=16) and chronic apical periodontitis (n=10) were eval-uated using flow cytometry. The PD-1 and PDL-1 expressions were evaluated using flow cytometry. The cy-tokine levels were evaluated by enzyme-linked immunosorbent assay. Data were analyzed using one-way ANOVA. The statistical significance level was set at P<0.05. Results: Results showed that the apical periodontitis lesions are more infiltrated by PD-1 + and PDL1 + lym-phocytes than the control samples. In addition, the PDL-1 expression was detected on macrophages in the apical periodontitis lesions, and was significantly higher compared to leukocytes from healthy gingival tis-sue. The IFN-γ, TGF-β, IL-10, and TNF-α levels were significantly higher in the apical periodontitis lesions com-pared to control samples. Conclusion: The PD-1, PD-L1, and CTLA-4 molecules are evident in apical periodontitis, and can be an impor-tant immune checkpoint in chronic periapical periodontitis. Competing Interests: Disclosures Conflict of interest: The authors declare that they have no conflicts of interest. (Copyright: © 2019 European Endodontic Journal.)
Databáze:	MEDLINE
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