Application of monoclonal antibodies in quantifying fungal growth dynamics during aerobic spoilage of silage.

Autor: Le Cocq K; Rothamsted Research, North Wyke, Okehampton, Devon, EX20 2SB, UK., Brown B; Rothamsted Research, North Wyke, Okehampton, Devon, EX20 2SB, UK., Hodgson CJ; Rothamsted Research, North Wyke, Okehampton, Devon, EX20 2SB, UK., McFadzean J; Rothamsted Research, North Wyke, Okehampton, Devon, EX20 2SB, UK., Horrocks CA; Rothamsted Research, North Wyke, Okehampton, Devon, EX20 2SB, UK., Lee MRF; Rothamsted Research, North Wyke, Okehampton, Devon, EX20 2SB, UK.; Bristol Veterinary School, University of Bristol, Langford, Somerset, BS40 5DU, UK., Davies DR; Rothamsted Research, North Wyke, Okehampton, Devon, EX20 2SB, UK.; Silage Solutions Ltd. Bwlch y Blaen, Pontrhdygroes, Ystrad Meurig, Ceredigion, SY25 6DP, UK.
Jazyk: angličtina
Zdroj: Microbial biotechnology [Microb Biotechnol] 2020 Jul; Vol. 13 (4), pp. 1054-1065. Date of Electronic Publication: 2020 Mar 10.
DOI: 10.1111/1751-7915.13552
Abstrakt: Proliferation of filamentous fungi following ingress of oxygen to silage is an important cause of dry matter losses, resulting in significant waste. In addition, the production of mycotoxins by some filamentous fungi poses a risk to animal health through mycotoxicosis. Quantitative assessment of fungal growth in silage, through measurement of ergosterol content, colony-forming units or temperature increase is limiting in representing fungal growth dynamics during aerobic spoilage due to being deficient in either representing fungal biomass or being able to identify specific genera. Here, we conducted a controlled environment aerobic exposure experiment to test the efficacy of a monoclonal antibody-based enzyme-linked immunosorbent assay (ELISA) to detect the proliferation of fungal biomass in six silage samples. We compared this to temperature which has been traditionally deployed in such experiments and on-farm to detect aerobic deterioration. In addition, we quantified ergosterol, a second marker of fungal biomass. After 8 days post-aerobic exposure, the ergosterol and ELISA methods indicated an increase in fungal biomass in one of the samples with a temperature increase observed after 16 days. A comparison of the methods with Pearson's correlation coefficient showed a positive association between temperature and ergosterol and both markers of fungal biomass. This work indicates that the technology has potential to be used as an indicator of microbial degradation in preserved forage. Consequently, if it developed as an on-farm technique, this could inform forage management decisions made by farmers, with the goal of decreasing dry matter losses, improving resource and nutrient efficiency and reducing risks to animal health.
(© 2020 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.)
Databáze: MEDLINE
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