Mycobacterium hassiacum: a thermophilic Mycobacterium species to demonstrate thermal disinfection of medical devices.

Autor: Haas B; STERIS Canada ULC, 490, Boulevard Armand-Paris, Quebec City, QC, G1C 8A3, Canada. Bruno_Haas@steris.com., Soto KJ; STERIS Corporation, Mentor, OH, USA., Day DS; STERIS Corporation, Mentor, OH, USA., Roy AC; STERIS Corporation, Mentor, OH, USA., Gagnon MC; STERIS Canada ULC, 490, Boulevard Armand-Paris, Quebec City, QC, G1C 8A3, Canada., Alt JR; STERIS Corporation, Mentor, OH, USA., Labrie P; STERIS Canada ULC, 490, Boulevard Armand-Paris, Quebec City, QC, G1C 8A3, Canada.
Jazyk: angličtina
Zdroj: BMC research notes [BMC Res Notes] 2020 Mar 10; Vol. 13 (1), pp. 140. Date of Electronic Publication: 2020 Mar 10.
DOI: 10.1186/s13104-020-04978-7
Abstrakt: Objective: Reprocessing reusable medical devices is crucial in the healthcare industry. To ensure patient safety, strict standards are dictated to validate thermal disinfection in automated washer-disinfectors. The United States Food and Drug Administration (FDA) has specific recommendations on the vegetative bacterial challenge but comparatively vague guidance on the use of a thermophilic Mycobacterium strain for thermal disinfection studies. This study aims to compare thermal resistance of Mycobacterium hassiacum and Mycobacterium terrae and determine which strain is suitable for medical device thermal disinfection validation testing in automated washer-disinfectors.
Results: Thermal resistance was demonstrated in vitro by calculating D-values for each strain at different exposure temperatures, and correlated with actual in situ processing conditions. M. terrae was completely killed (> 7 log reduction) at temperatures above 68 °C, with D-values between 46.6 and 27.8 s at temperatures between 59.5 and 67.2 °C. M. hassiacum was completely killed (> 8 log reduction) at temperatures above 75 °C, with D-values between 82.1 and 21.7 s at temperatures ranging between 69.2 and 73.6 °C. In vitro results were correlated in a washer-disinfector performance validation setup.
Databáze: MEDLINE
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