Comparison of the Effect of Platinum (IV) Complexes on Spheroids and Monolayer Culture of HeLa Cells.

Autor: Trigub ММ; Institute of Problems of Chemical Physics, Russian Academy of Science, Chernogolovka, Moscow, Russia. trigub.m@gmail.com.; Scientific and Educational Center of the Moscow State Regional University in Chernogolovka, Mytishchi, Moscow, Russia. trigub.m@gmail.com., Filatova NV; Institute of Problems of Chemical Physics, Russian Academy of Science, Chernogolovka, Moscow, Russia.; Scientific and Educational Center of the Moscow State Regional University in Chernogolovka, Mytishchi, Moscow, Russia., Areshidze DА; Institute of Problems of Chemical Physics, Russian Academy of Science, Chernogolovka, Moscow, Russia.; Scientific and Educational Center of the Moscow State Regional University in Chernogolovka, Mytishchi, Moscow, Russia.; M. V. Lomonosov Moscow State University, Moscow, Russia., Sen' VD; Institute of Problems of Chemical Physics, Russian Academy of Science, Chernogolovka, Moscow, Russia., Terentiev АА; Institute of Problems of Chemical Physics, Russian Academy of Science, Chernogolovka, Moscow, Russia.; Scientific and Educational Center of the Moscow State Regional University in Chernogolovka, Mytishchi, Moscow, Russia.; M. V. Lomonosov Moscow State University, Moscow, Russia.
Jazyk: angličtina
Zdroj: Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2020 Feb; Vol. 168 (4), pp. 583-588. Date of Electronic Publication: 2020 Mar 09.
DOI: 10.1007/s10517-020-04757-1
Abstrakt: We performed a comparative study of the cytotoxicity of cisplatin, JM216 complex, and aminonitroxyl platinum(IV) complexes for HeLa cells grown in monolayer and 3D culture. The growth dynamics of spheroids was studied and optimal conditions for evaluation of cytotoxicity were determined. Spheroids were less sensitive to the test compounds than cells in a monolayer. The resistance index (RI) of spheroids was determined as the ratio of IC 50 for spheroids to IC 50 for monolayer culture. Resistance index was 5.0±1.5 for cisplatin and ranged from 1.8 to 2.3 for platinum(IV) complexes. The observed differences are related to different physicochemical properties of the complexes and different mechanisms of their penetration into cells.
Databáze: MEDLINE
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