Cytokinetic bridge triggers de novo lumen formation in vivo.

Autor: Rathbun LI; Biology Department, Syracuse University, Syracuse, New York, USA., Colicino EG; Biology Department, Syracuse University, Syracuse, New York, USA.; Department of Cell and Developmental Biology, SUNY Upstate Medical School, Syracuse, New York, USA.; Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, Michigan, USA., Manikas J; Biology Department, Syracuse University, Syracuse, New York, USA., O'Connell J; Biology Department, Syracuse University, Syracuse, New York, USA., Krishnan N; Biology Department, Syracuse University, Syracuse, New York, USA., Reilly NS; Department of Physics and Astronomy, University of Rochester, Rochester, New York, USA., Coyne S; Department of Cell and Developmental Biology, SUNY Upstate Medical School, Syracuse, New York, USA.; Department of Biology, SUNY Geneseo, Geneseo, New York, USA., Erdemci-Tandogan G; Department of Physics, Syracuse University, Syracuse, New York, USA., Garrastegui A; Biology Department, Syracuse University, Syracuse, New York, USA., Freshour J; Biology Department, Syracuse University, Syracuse, New York, USA., Santra P; Department of Cell and Developmental Biology, SUNY Upstate Medical School, Syracuse, New York, USA., Manning ML; Department of Physics, Syracuse University, Syracuse, New York, USA., Amack JD; Department of Cell and Developmental Biology, SUNY Upstate Medical School, Syracuse, New York, USA., Hehnly H; Biology Department, Syracuse University, Syracuse, New York, USA. hhehnly@syr.edu.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2020 Mar 09; Vol. 11 (1), pp. 1269. Date of Electronic Publication: 2020 Mar 09.
DOI: 10.1038/s41467-020-15002-8
Abstrakt: Multicellular rosettes are transient epithelial structures that serve as intermediates during diverse organ formation. We have identified a unique contributor to rosette formation in zebrafish Kupffer's vesicle (KV) that requires cell division, specifically the final stage of mitosis termed abscission. KV utilizes a rosette as a prerequisite before forming a lumen surrounded by ciliated epithelial cells. Our studies identify that KV-destined cells remain interconnected by cytokinetic bridges that position at the rosette's center. These bridges act as a landmark for directed Rab11 vesicle motility to deliver an essential cargo for lumen formation, CFTR (cystic fibrosis transmembrane conductance regulator). Here we report that premature bridge cleavage through laser ablation or inhibiting abscission using optogenetic clustering of Rab11 result in disrupted lumen formation. We present a model in which KV mitotic cells strategically place their cytokinetic bridges at the rosette center, where Rab11-associated vesicles transport CFTR to aid in lumen establishment.
Databáze: MEDLINE