Compound isolation and biological activities of Piptadeniastrum africanum (hook.f.) Brennan roots.
| Autor: | Mbosso Teinkela JE; Microbiology, Bioorganic and Macromolecular Chemistry Unit, Department RD3, Faculté de Pharmacie, Université Libre de Bruxelles (ULB), Belgium; Department of Biological Sciences, Faculty of Medicine and Pharmaceutical Sciences, University of Douala, Cameroon; Department of Chemistry, Faculty of Science, University of Douala, Douala, Cameroon. Electronic address: embosso@yahoo.fr., Siwe Noundou X; University Institute of Wood Technology of Mbalmayo, University of Yaoundé 1, Cameroon; Nanomaterials and Medicinal Organic Chemistry Laboratory, Department of Chemistry, Faculty of Science, Rhodes University, South Africa; Department of Biochemistry and Microbiology, Rhodes University, South Africa., Zeh Mimba JE; Department of Chemistry, Faculty of Science, University of Douala, Douala, Cameroon., Meyer F; Microbiology, Bioorganic and Macromolecular Chemistry Unit, Department RD3, Faculté de Pharmacie, Université Libre de Bruxelles (ULB), Belgium., Tabouguia OM; Programme in Medicine, Faculty of Health Science, University of Buea, Cameroon., Assob Nguedia JC; Programme in Medicine, Faculty of Health Science, University of Buea, Cameroon., Hoppe HC; Department of Biochemistry and Microbiology, Rhodes University, South Africa., Krause RWM; Nanomaterials and Medicinal Organic Chemistry Laboratory, Department of Chemistry, Faculty of Science, Rhodes University, South Africa., Wintjens R; Microbiology, Bioorganic and Macromolecular Chemistry Unit, Department RD3, Faculté de Pharmacie, Université Libre de Bruxelles (ULB), Belgium., Azebaze GAB; Department of Chemistry, Faculty of Science, University of Douala, Douala, Cameroon. |
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| Jazyk: | angličtina |
| Zdroj: | Journal of ethnopharmacology [J Ethnopharmacol] 2020 Jun 12; Vol. 255, pp. 112716. Date of Electronic Publication: 2020 Mar 06. |
| DOI: | 10.1016/j.jep.2020.112716 |
| Abstrakt: | Ethnopharmacological Relevance: The dicotyledonous plant Piptadeniastrum africanum (hook.f.) Brennan (Fabaceae) is used in traditional medicine to treat various human complaints including bronchitis, coughing, urino-genital ailments, meningitis, abdominal pain, treatment of wounds, malaria and gastrointestinal ailments, and is used as a purgative and worm expeller. Aim of the Study: The present study describes the phytochemical investigation and the determination of the antimicrobial, antiplasmodial and antitrypanosomal activities of crude extract, fractions and compounds extracted from Piptadeniastrum africanum roots. Materials and Methods: Isolated compounds were obtained using several chromatographic techniques. The structures of all compounds were determined by comprehensive spectroscopic analyses (1D and 2D NMR) and by comparing their NMR data with those found in literature. In vitro antimicrobial activity of samples was evaluated using the microdilution method on bacterial (Escherichia coli, Proteus mirabilis, Staphylococcus aureus) and fungal (Candida krusei) strains, while in vitro cell-growth inhibition activities were assessed against two parasites (Trypanosoma brucei brucei and Plasmodium falciparum strain 3D7). The cytotoxicity properties of samples were assayed against HeLa human cervical carcinoma. Results: Five compounds were isolated and identified as: tricosanol 1, 5α-stigmasta-7,22-dien-3-β-ol 2, betulinic acid 3, oleanolic acid 4 and piptadenamide 5. This is the first report of the isolation of these five compounds from the roots of P. africanum. The (Hex:EtOAc 50:50) fraction exhibited moderate antibacterial activity against P. mirabilis (MIC 250 μg/mL), while the other fractions and isolated compounds had weak antimicrobial activities. Only the EtOAc fraction presented a moderate antimalarial activity with an IC Conclusion: These results demonstrated a scientific rational of the traditional uses of P. africanum and indicate that this plant should be further investigated to identify some of the chemical components that exhibited the activities reported in this study and therefore may constitute new lead candidates in parasiticidal drug discovery. Competing Interests: Declaration of competing interest There is no competing interest among the authors. (Copyright © 2020 Elsevier B.V. All rights reserved.) |
| Databáze: | MEDLINE |
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