The T102C polymorphism of 5HT2A receptor in oral epithelial dysplasia: A pilot case-control study.

Autor: Severo RF; Graduate Program in Health and Behavior, Catholic University of Pelotas, Pelotas, RS, 96010-901, Brazil., do Amaral CC; Graduate Program in Health and Behavior, Catholic University of Pelotas, Pelotas, RS, 96010-901, Brazil., Garcia TF; Graduate Program in Health and Behavior, Catholic University of Pelotas, Pelotas, RS, 96010-901, Brazil., Ferrúa CP; Graduate Program in Health and Behavior, Catholic University of Pelotas, Pelotas, RS, 96010-901, Brazil., Corrêa GP; Graduate Program in Health and Behavior, Catholic University of Pelotas, Pelotas, RS, 96010-901, Brazil., Klug AB; Graduate Program in Health and Behavior, Catholic University of Pelotas, Pelotas, RS, 96010-901, Brazil., da Silva KD; Graduate Program in Dentistry of the Federal University of Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil., Bastos CR; Graduate Program in Health and Behavior, Catholic University of Pelotas, Pelotas, RS, 96010-901, Brazil., Britto Correa M; Graduate Program in Dentistry of the Federal University of Pelotas, Pelotas, RS, 96010-610, Brazil., Ghisleni GC; Graduate Program in Health and Behavior, Catholic University of Pelotas, Pelotas, RS, 96010-901, Brazil., Uchoa Vasconcelos AC; Graduate Program in Dentistry of the Federal University of Pelotas, Pelotas, RS, 96010-610, Brazil., Tarquinio SBC; Graduate Program in Dentistry of the Federal University of Pelotas, Pelotas, RS, 96010-610, Brazil., Nedel F; Graduate Program in Health and Behavior, Catholic University of Pelotas, Pelotas, RS, 96010-901, Brazil. Electronic address: fernanda.nedel@gmail.com.
Jazyk: angličtina
Zdroj: Archives of oral biology [Arch Oral Biol] 2020 May; Vol. 113, pp. 104688. Date of Electronic Publication: 2020 Feb 24.
DOI: 10.1016/j.archoralbio.2020.104688
Abstrakt: Objective: investigate the T102C polymorphism of 5HT2A receptor in dysplasia in oral potentially malignant lesions and its association with smoking and alcohol habits.
Methods: case-control study that included patients with oral potentially malignant lesions (OPML) histopathologically diagnosed with dysplasia and healthy controls, and within these group patients with and without smoking and alcohol consumption habits. Cell samples from the oral lesions were collected with the patients previously anesthetized using disposable cytological brushes. Deoxyribonucleic acid (DNA) extraction was performed and the T102C polymorphism (rs6313) was genotyped in a real-time polymerase chain reaction (PCR) allelic discrimination assays.
Results: 110 individuals were included in this study (38 with dysplasia and 72 controls). The genotype (p = 0.016), allele (p = 0.020) and smoking habits (<0.001) distribution differed significantly between dysplasia and control group, where the CT and TT (C - cytosine/ T - thymine) genotype and the T allele showed a higher frequency in dysplasia (65.6, 18.8 and 84.4 %, respectively) than in controls (55.7, 4.9 and 60.7). Concerning smoking habits, the higher frequency was in the dysplasia group. The multivariate logistic regression analysis, associating variables of interest and the presence of dysplasia, showed that individuals with smoking habits present 7.58 increase risk to develop dysplasia than non-smokers; and individuals carrying the T allele for the T102C polymorphism have a 4.6 increased risk to develop oral dysplasia in OPML.
Conclusions: the T102C polymorphism is associated with oral dysplasia in OPML, however, failed to show association with smoking and alcohol habits in OPML dysplasia.
Competing Interests: Declaration of Competing Interest None.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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