Development of a 3D human osteoblast cell culture model for studying mechanobiology in orthodontics.

Autor:	Brezulier D; Univ Rennes, CHU Rennes, Pole Odontologie, Rennes, France.; ISCR, CNRS-UMR 6226, Rennes, France., Pellen-Mussi P; ISCR, CNRS-UMR 6226, Rennes, France., Tricot-Doleux S; ISCR, CNRS-UMR 6226, Rennes, France., Novella A; ISCR, CNRS-UMR 6226, Rennes, France., Sorel O; Univ Rennes, CHU Rennes, Pole Odontologie, Rennes, France., Jeanne S; Univ Rennes, CHU Rennes, Pole Odontologie, Rennes, France.; ISCR, CNRS-UMR 6226, Rennes, France.
Jazyk:	angličtina
Zdroj:	European journal of orthodontics [Eur J Orthod] 2020 Sep 11; Vol. 42 (4), pp. 387-395.
DOI:	10.1093/ejo/cjaa017
Abstrakt:	Objectives: Mechanobiology phenomena constitute a major element of the cellular and tissue response during orthodontic treatment and the implantation of a biomaterial. Better understanding these phenomena will improve the effectiveness of our treatments. The objective of this work is to validate a model of three-dimensional (3D) culture of osteoblasts to study mechanobiology. Materials and Methods: The hFOB 1.19 cell line was cultured either traditionally on a flat surface or in aggregates called spheroids. They were embedded in 0.8% low-melting agarose type VII and placed in a polyethylene terephthalate transwell insert. Compressive forces of 1 and 4 g/cm2 were applied with an adjustable weight. Proliferation was evaluated by measuring diameters, monitoring glucose levels, and conducting Hoechst/propidium iodide staining. Enzyme-linked immunosorbent assays focusing on the pro-inflammatory mediators interleukin (IL)-6 and IL-8 and bone remodelling factor osteoprotegerin were performed to evaluate soluble factor synthesis. quantitative reverse transcription-polymerase chain reaction was performed to evaluate bone marker transcription. Results: The 3D model shows good cell viability and permits IL dosing. Additionally, three gene expression profiles are analysable. Limitations: The model allows analysis of conventional markers; larger exploration is needed for better understanding osteoblast mechanobiology. However, it only allows an analysis over 3 days. Conclusion: The results obtained by applying constant compressive forces to 3D osteoblastic cultures validate this model system for exploring biomolecule release and analysing gene transcription. In particular, it highlights a disturbance in the expression of markers of osteogenesis. (© The Author(s) 2020. Published by Oxford University Press on behalf of the European Orthodontic Society. All rights reserved. For permissions, please email: journals.permissions@oup.com.)
Databáze:	MEDLINE
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