Peroxiredoxin 2 deficiency does not affect insulin resistance and oxidative stress in high-fat diet-fed obese mice.

Autor: Kim JH; Department of Urology, Soon Chun Hyung University Hospital, Gumi, Korea.; Department of Physiology, College of Medicine, Yeungnam University, Daegu, Korea., Cha HN; Department of Physiology, College of Medicine, Yeungnam University, Daegu, Korea.; Smart-aging Convergence Research Center, College of Medicine, Yeungnam University, Daegu, Korea., Kim YW; Department of Physiology, College of Medicine, Yeungnam University, Daegu, Korea., Park SY; Department of Physiology, College of Medicine, Yeungnam University, Daegu, Korea.; Smart-aging Convergence Research Center, College of Medicine, Yeungnam University, Daegu, Korea.
Jazyk: angličtina
Zdroj: Archives of physiology and biochemistry [Arch Physiol Biochem] 2022 Aug; Vol. 128 (4), pp. 859-868. Date of Electronic Publication: 2020 Mar 06.
DOI: 10.1080/13813455.2020.1733026
Abstrakt: Objective: To examine if peroxiredoxin 2 (Prx2) deficiency aggravates high-fat diet-induced insulin resistance.
Material and Methods: Insulin sensitivity was measured in Prx2 knockout (KO) and wild-type (WT) littermates using the hyperinsulinemic-euglycemic clamp.
Results: Whole body glucose turnover, glucose uptake, and levels of glucose transporter 4 (Glut4) protein in the skeletal muscle were found to be lower. This was followed by increased expression of oxidative stress markers in Prx2 KO mice than that in WT mice in the control diet group. Although, a 12-week high-fat diet induced insulin resistance and enhanced oxidative stress in both genotypes, there was no difference between WT and Prx2 KO mice with respect to insulin sensitivity and the level of oxidative stress markers. Accordingly, the levels of phosphorylated Akt and Glut4 were similar between the two genotypes.
Conclusion: These results suggest that Prx2 does not affect high-fat diet-induced oxidative stress and insulin resistance in mice.
Databáze: MEDLINE
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