Microenvironment mapping via Dexter energy transfer on immune cells.

Autor: Geri JB; Merck Center for Catalysis, Princeton University, Princeton, NJ 08544, USA., Oakley JV; Merck Center for Catalysis, Princeton University, Princeton, NJ 08544, USA., Reyes-Robles T; Merck Exploratory Science Center, Merck & Co., Inc., Cambridge, MA 02141, USA., Wang T; Merck Center for Catalysis, Princeton University, Princeton, NJ 08544, USA., McCarver SJ; Merck Center for Catalysis, Princeton University, Princeton, NJ 08544, USA., White CH; Merck Exploratory Science Center, Merck & Co., Inc., Cambridge, MA 02141, USA., Rodriguez-Rivera FP; Discovery Chemistry, Merck & Co., Inc., Kenilworth, NJ 07033, USA., Parker DL Jr; Discovery Chemistry, Merck & Co., Inc., Kenilworth, NJ 07033, USA., Hett EC; Merck Exploratory Science Center, Merck & Co., Inc., Cambridge, MA 02141, USA., Fadeyi OO; Merck Exploratory Science Center, Merck & Co., Inc., Cambridge, MA 02141, USA. olugbeminiyi.fadeyi@merck.com rob.oslund@merck.com dmacmill@princeton.edu., Oslund RC; Merck Exploratory Science Center, Merck & Co., Inc., Cambridge, MA 02141, USA. olugbeminiyi.fadeyi@merck.com rob.oslund@merck.com dmacmill@princeton.edu., MacMillan DWC; Merck Center for Catalysis, Princeton University, Princeton, NJ 08544, USA. olugbeminiyi.fadeyi@merck.com rob.oslund@merck.com dmacmill@princeton.edu.
Jazyk: angličtina
Zdroj: Science (New York, N.Y.) [Science] 2020 Mar 06; Vol. 367 (6482), pp. 1091-1097.
DOI: 10.1126/science.aay4106
Abstrakt: Many disease pathologies can be understood through the elucidation of localized biomolecular networks, or microenvironments. To this end, enzymatic proximity labeling platforms are broadly applied for mapping the wider spatial relationships in subcellular architectures. However, technologies that can map microenvironments with higher precision have long been sought. Here, we describe a microenvironment-mapping platform that exploits photocatalytic carbene generation to selectively identify protein-protein interactions on cell membranes, an approach we term MicroMap (μMap). By using a photocatalyst-antibody conjugate to spatially localize carbene generation, we demonstrate selective labeling of antibody binding targets and their microenvironment protein neighbors. This technique identified the constituent proteins of the programmed-death ligand 1 (PD-L1) microenvironment in live lymphocytes and selectively labeled within an immunosynaptic junction.
(Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.)
Databáze: MEDLINE
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