Positive staining of the immunoligand B7-H6 in abnormal/transformed keratinocytes consistently accompanies the progression of cervical cancer.

Autor: Gutierrez-Silerio GY; Instituto de Enfermedades Crónico Degenerativas, Departamento de Biología Molecular y Genómica, CUCS, Universidad de Guadalajara, Sierra Mojada # 950, Colonia Independencia, CP 44340, Guadalajara, Jalisco, Mexico.; Instituto Transdisciplinar de Investigación y Servicios, CUCEI, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico., Franco-Topete RA; Laboratorio de Patología, Departamento de Microbiología y Patología, CUCS, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico.; Departamento de Anatomía Patológica, Nuevo Hospital Civil de Guadalajara 'Dr. Juan I. Menchaca', Guadalajara, Jalisco, Mexico., Haramati J; Laboratorio de Inmunobiología, Departamento de Biología Celular y Molecular, CUCBA, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico., Navarrete-Medina EM; Laboratorio de Patología, Departamento de Microbiología y Patología, CUCS, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico.; Departamento de Anatomía Patológica, Nuevo Hospital Civil de Guadalajara 'Dr. Juan I. Menchaca', Guadalajara, Jalisco, Mexico., Gutierrez-Franco J; Unidad Académica de Ciencias Químico Biológicas y Farmacéuticas, Universidad Autónoma de Nayarit, Tepic, Nayarit, Mexico., Bueno-Topete MR; Instituto de Enfermedades Crónico Degenerativas, Departamento de Biología Molecular y Genómica, CUCS, Universidad de Guadalajara, Sierra Mojada # 950, Colonia Independencia, CP 44340, Guadalajara, Jalisco, Mexico.; Instituto Transdisciplinar de Investigación y Servicios, CUCEI, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico., Bastidas-Ramirez BE; Instituto de Enfermedades Crónico Degenerativas, Departamento de Biología Molecular y Genómica, CUCS, Universidad de Guadalajara, Sierra Mojada # 950, Colonia Independencia, CP 44340, Guadalajara, Jalisco, Mexico.; Instituto Transdisciplinar de Investigación y Servicios, CUCEI, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico., Ramos-Marquez ME; Instituto de Enfermedades Crónico Degenerativas, Departamento de Biología Molecular y Genómica, CUCS, Universidad de Guadalajara, Sierra Mojada # 950, Colonia Independencia, CP 44340, Guadalajara, Jalisco, Mexico., Del Toro-Arreola S; Instituto de Enfermedades Crónico Degenerativas, Departamento de Biología Molecular y Genómica, CUCS, Universidad de Guadalajara, Sierra Mojada # 950, Colonia Independencia, CP 44340, Guadalajara, Jalisco, Mexico. susana@cucs.udg.mx.; Instituto Transdisciplinar de Investigación y Servicios, CUCEI, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico. susana@cucs.udg.mx.; Laboratorio de Inmunología, Departamento de Fisiología, CUCS, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico. susana@cucs.udg.mx.
Jazyk: angličtina
Zdroj: BMC immunology [BMC Immunol] 2020 Mar 06; Vol. 21 (1), pp. 9. Date of Electronic Publication: 2020 Mar 06.
DOI: 10.1186/s12865-020-0341-9
Abstrakt: Background: B7-H6 has been revealed as an endogenous immunoligand expressed in a variety of tumors, but not expressed in healthy tissues. Heretofore, no studies have been reported describing B7-H6 in women with cervical cancer. To investigate this question, our present study was conducted.
Results: This retrospective study comprised a total of 62 paraffinized cervical biopsies, which were distributed in five groups: low-grade squamous intraepithelial lesions (LSIL), high-grade squamous intraepithelial lesions (HSIL), squamous cervical carcinoma (SCC), uterine cervical adenocarcinoma (UCAC), and a group of cervicitis (as a control for non-abnormal/non-transformed cells). Cervical sections were stained by immunohistochemistry to explore the expression of B7-H6, which was reported according to the immunoreactive score (IRS) system. We observed a complete lack of B7-H6 in LSIL abnormal epithelial cells. Interestingly, B7-H6 began to be seen in HSIL abnormal epithelial cells; more than half of this group had B7-H6 positive cells, with staining characterized by a cytoplasmic and membranous pattern. B7-H6 in the SCC group was also seen in the majority of the sections, showing the same cytoplasmic and membranous pattern. Strong evidence of B7-H6 was notably found in UCAC tumor columnar cells (in 100% of the specimens, also with cytoplasmic and membranous pattern). Moreover, consistent B7-H6 staining was observed in infiltrating plasma cells in all groups.
Conclusions: B7-H6 IRS positively correlated with disease stage in the development of cervical cancer; additionally, B7-H6 scores were found to be even higher in the more aggressive uterine cervical adenocarcinoma, suggesting a possible future therapeutic target for this cancer type.
Databáze: MEDLINE
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