Intra-articular injection of 2-pyridylethylamine produces spinal NPY-mediated antinociception in the formalin-induced rat knee-joint pain model.

Autor: Souza-Silva E; Departamento de Farmacologia, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, 88049-970 Florianópolis, Santa Catarina, Brazil., Stein T; Departamento de Farmacologia, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, 88049-970 Florianópolis, Santa Catarina, Brazil., Mascarin LZ; Departamento de Farmacologia, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, 88049-970 Florianópolis, Santa Catarina, Brazil., Dornelles FN; Departamento de Farmacologia, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, 88049-970 Florianópolis, Santa Catarina, Brazil., Bicca MA; Departamento de Farmacologia, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, 88049-970 Florianópolis, Santa Catarina, Brazil., Tonussi CR; Departamento de Farmacologia, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, 88049-970 Florianópolis, Santa Catarina, Brazil. Electronic address: c.r.tonussi@ufsc.br.
Jazyk: angličtina
Zdroj: Brain research [Brain Res] 2020 May 15; Vol. 1735, pp. 146757. Date of Electronic Publication: 2020 Mar 02.
DOI: 10.1016/j.brainres.2020.146757
Abstrakt: Low doses of histamine or H1R agonist 2-pyridylethylamine (2-PEA) into the knee-joint were found to decrease formalin-induced articular nociception in rats. In this study, we evaluated the participation of spinal NPY in the antinociceptive effect produced by 2-PEA. Injection of formalin (1.5%) into one of the knee-joints causes the limping of the respective limb due to nociception, which was registered each 5 min over 60 min. Neuropeptide Y1 receptor (Y1R) content in the spinal cord was evaluated by western-blotting. Intrathecal (i.t.) injection of Y1R agonist Leu31, Pro34-NPY (0.7-7 µmol) decreased nociception, while injection of the antagonist BIBO 3304 (4 μmol), increased nociception. Antinociception produced by 2-PEA was reversed by a sub-effective i.t. dose of the Y1R antagonist. Similarly, this antinociceptive effect was prevented by i.t. pretreatment with the neurotoxin NPY-saporin (750 ng), which also reduced immunoblotting for Y1R in spinal cord homogenates. These data support the idea that antinociception induced by H1R agonists in the knee-joint of rats may be mediated by the spinal release of NPY, and this peptide seems to be acting via Y1R.
(Copyright © 2020. Published by Elsevier B.V.)
Databáze: MEDLINE
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