Effects of Gamma-Radiation on DNA Damage in Onion (Allium cepa L.) Seedlings.

Autor: Bolsunovsky AY; Institute of Biophysics, Krasnoyarsk Science Center, Siberian Branch, Russian Academy of Sciences, 660036, Krasnoyarsk, Russia. radecol@ibp.ru., Dementyev DV; Institute of Biophysics, Krasnoyarsk Science Center, Siberian Branch, Russian Academy of Sciences, 660036, Krasnoyarsk, Russia., Frolova TS; Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, 630090, Novosibirsk, Russia.; Novosibirsk State University, 630090, Novosibirsk, Russia., Trofimova EA; Institute of Biophysics, Krasnoyarsk Science Center, Siberian Branch, Russian Academy of Sciences, 660036, Krasnoyarsk, Russia., Iniatkina EM; Institute of Biophysics, Krasnoyarsk Science Center, Siberian Branch, Russian Academy of Sciences, 660036, Krasnoyarsk, Russia., Vasilyev SA; Research Institute of Medical Genetics, Tomsk National Research Medical Center, Russian Academy of Sciences, 634058, Tomsk, Russia., Sinitsyna OI; Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, 630090, Novosibirsk, Russia.; Novosibirsk State University, 630090, Novosibirsk, Russia.
Jazyk: angličtina
Zdroj: Doklady. Biochemistry and biophysics [Dokl Biochem Biophys] 2019 Nov; Vol. 489 (1), pp. 362-366. Date of Electronic Publication: 2020 Mar 04.
DOI: 10.1134/S1607672919060024
Abstrakt: The effect of γ-radiation on the level of nuclear DNA damage in onion seedlings (Allium-test) was studied using the comet assay. DNA breaks were first found in cells of onion seedlings exposed to low-dose radiation (≤ 0.1 Gy). Dose dependence of DNA damage parameters showed nonlinear behavior: a linear section in the low-dose region (below 0.1 Gy) and a dose-independent plateau in the dose range between 1 and 5 Gy. Thus, the comet assay can be used to estimate the biological effects of low-dose γ-radiation on Allium cepa seedlings.
Databáze: MEDLINE
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