Optimized germinated soybean/cornstarch extrudate and its in vitro fermentation with human inoculum.

Autor: Cruz-Ortiz R; 1Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Unidad Profesional Adolfo López Mateos, Av. Wilfrido Massieu, 399, C.P. 07738 Ciudad de México, CDMX Mexico., Gutiérrez-Dorado R; 2Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Sinaloa, Culiacán, Sin. Mexico., Osorio-Díaz P; 3Centro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional (CEPROBI-IPN), Carretera Yautepec-Jojutla, Km. 6, calle CEPROBI No. 8, Col. San Isidro, C.P. 62731 Yautepec, Morelos Mexico., Alvarado-Jasso GM; 3Centro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional (CEPROBI-IPN), Carretera Yautepec-Jojutla, Km. 6, calle CEPROBI No. 8, Col. San Isidro, C.P. 62731 Yautepec, Morelos Mexico., Tornero-Martínez A; 1Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Unidad Profesional Adolfo López Mateos, Av. Wilfrido Massieu, 399, C.P. 07738 Ciudad de México, CDMX Mexico., Guzmán-Ortiz FA; 4CONACYT-Universidad Autónoma del Estado de Hidalgo, Carretera Pachuca-Tulancingo, Km 4.5, Ciudad del Conocimiento, 42184 Mineral de la Reforma, Hidalgo Mexico., Mora-Escobedo R; 1Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Unidad Profesional Adolfo López Mateos, Av. Wilfrido Massieu, 399, C.P. 07738 Ciudad de México, CDMX Mexico.
Jazyk: angličtina
Zdroj: Journal of food science and technology [J Food Sci Technol] 2020 Mar; Vol. 57 (3), pp. 848-857. Date of Electronic Publication: 2019 Oct 03.
DOI: 10.1007/s13197-019-04116-7
Abstrakt: The aim of this work was to optimise a soybean/cornstarch extrudate by adjusting a central composite design and to maximise a product with a high protein and resistant starch (RS) content by evaluating the indigestible fractions through in vitro colonic fermentation and production of short-chain fatty acids (SCFAs) with potential health benefits. According to the response surface analysis and RS maximisation results, an optimisation of the independent variables was obtained as follows: 32.5% feed moisture, 144 °C extrusion temperature and a proportion of 44% germinated soybean flour and 56% cornstarch. A product with a 2.11% expansion index, 6.25 N hardness, a glycaemic index of 49 and 12% resistant starch was obtained. The optimised extrudate showed a 36% indigestible fraction and high fermentability with respect to that of the lactulose control. Furthermore, the decrease in pH was inversely proportional to the production of SCFAs and the volume of gas generated. Acetic, propionic, and butyric acids were produced at a molar ratio of 62:27:11, while the highest SCFA concentrations were found 48 h after incubation. The RS of the optimised extruder was a viable substrate for in vitro colonic fermentation, suggesting that it is a good food source to produce SCFAs, which could exert an effect on the regulation of lipid and glucose metabolism.
(© Association of Food Scientists & Technologists (India) 2019.)
Databáze: MEDLINE