A Survey of Antimicrobial Resistance Determinants in Category A Select Agents, Exempt Strains, and Near-Neighbor Species.

Autor: Taitt CR; US Naval Research Laboratory, Center for Biomolecular Science & Engineering, Washington, DC 20375, USA., Leski TA; US Naval Research Laboratory, Center for Biomolecular Science & Engineering, Washington, DC 20375, USA., Chen A; Karle's Fellow, US Naval Research Laboratory, Washington, DC 20375, USA., Berk KL; US Army Combat Capabilities Development Command (CCDC) Chemical Biological Center, Edgewood, MD 21010, USA., Dorsey RW; US Army Combat Capabilities Development Command (CCDC) Chemical Biological Center, Edgewood, MD 21010, USA., Gregory MJ; US Naval Medical Research Center - Biological Defense Research Directorate, Frederick, MD 20910, USA., Sozhamannan S; Defense Biological Product Assurance Office, Joint Program Executive Office for Chemical, Biological, Radiological and Nuclear Defense (CBRND), Frederick, MD 21702, USA.; Logistics Management Institute, Tysons, VA 22012, USA., Frey KG; US Naval Medical Research Center - Biological Defense Research Directorate, Frederick, MD 20910, USA., Dutt DL; Defense Threat Reduction Agency, Joint Science and Technology Office, Ft. Belvoir, VA 22060, USA., Vora GJ; US Naval Research Laboratory, Center for Biomolecular Science & Engineering, Washington, DC 20375, USA.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2020 Feb 29; Vol. 21 (5). Date of Electronic Publication: 2020 Feb 29.
DOI: 10.3390/ijms21051669
Abstrakt: A dramatic increase in global antimicrobial resistance (AMR) has been well documented. Of particular concern is the dearth of information regarding the spectrum and prevalence of AMR within Category A Select Agents. Here, we performed a survey of horizontally and vertically transferred AMR determinants among Category A agents and their near neighbors. Microarrays provided broad spectrum screening of 127 Francisella spp., Yersinia spp., and Bacillus spp. strains for the presence/absence of 500+ AMR genes (or families of genes). Detecting a broad variety of AMR genes in each genus, microarray analysis also picked up the presence of an engineered plasmid in a Y. pestis strain. High resolution melt analysis (HRMA) was also used to assess the presence of quinolone resistance-associated mutations in 100 of these strains. Though HRMA was able to detect resistance-causing point mutations in B. anthracis strains, it was not capable of discriminating these point mutations from other nucleotide substitutions (e.g., arising from sequence differences in near neighbors). Though these technologies are well-established, to our knowledge, this is the largest survey of Category A agents and their near-neighbor species for genes covering multiple mechanisms of AMR.
Competing Interests: The authors declare no conflict of interest. DTRA was active in both the research design and project management, and in preparation of the manuscript. This manuscript is approved for public release: distribution is unlimited.
Databáze: MEDLINE
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