Low myocardial transcript variant alt-a of cyclin dependent kinase inhibitor p21 expression differentiates hypothermia from cardiac/respiratory causes of death.

Autor:	Kaija H; Department of Forensic Medicine, Research Unit of Internal Medicine, Faculty of Medicine, Medical Research Center Oulu, Oulu University Hospital and University of Oulu., Pakanen L; Department of Forensic Medicine, Research Unit of Internal Medicine, Faculty of Medicine, Medical Research Center Oulu, Oulu University Hospital and University of Oulu.; Forensic Medicine Unit, National Institute for Health and Welfare., Porvari K; Department of Forensic Medicine, Research Unit of Internal Medicine, Faculty of Medicine, Medical Research Center Oulu, Oulu University Hospital and University of Oulu.; Department of Pathology, Research Unit of Cancer and Translational Medicine, Faculty of Medicine, Medical Research Center Oulu, Oulu University Hospital and University of Oulu, Finland.
Jazyk:	angličtina
Zdroj:	Medicine [Medicine (Baltimore)] 2020 Feb; Vol. 99 (9), pp. e19399.
DOI:	10.1097/MD.0000000000019399
Abstrakt:	Gene expressions in the myocardium have been shown to vary between different causes of death, which can be utilized in the recognition of varied processes. Our previous work with a limited number of cases showed a high messenger ribonucleic acid expression of the transcript variant alt-a of cyclin dependent kinase inhibitor p21 (p21 alt-a) in chronic cardiac ischemia deaths and a low expression in hypothermia deaths and acute myocardial ischemia deaths. In present work, p21 alt-a expression in the myocardium of human cadavers was calculated using glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as reference gene. In this collection of 143 samples, the p21 alt-a expression was significantly lower in hypothermia than in chronic cardiac ischemic heart disease with (P < .001) or without (P < .001) acute myocardial infarction and in other cardiac and respiratory disease deaths (P < .000). Chronic ischemic heart disease in hypothermia cases did not increase the expression. The p21 alt-a expression did not correlate with postmortem interval, quality of RNA or with the age of the deceased. The p21 alt-a referenced to GAPDH expression in cadaver myocardium has apparent potential as a marker distinguishing between hypothermia and cardiac/respiratory diseases as causes of death.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu