Relationship between energy balance and metabolic profiles in plasma and milk of dairy cows in early lactation.

Autor:	Xu W; Adaptation Physiology Group, Department of Animal Sciences, Wageningen University and Research, PO Box 338, 6700 AH, Wageningen, the Netherlands.; Laboratory of Biochemistry, Wageningen University and Research, PO Box 338, 6700 AH, Wageningen, the Netherlands., Vervoort J; Laboratory of Biochemistry, Wageningen University and Research, PO Box 338, 6700 AH, Wageningen, the Netherlands., Saccenti E; Laboratory of Systems and Synthetic Biology, Wageningen University and Research, Stippeneng 4, 6708 WE, Wageningen, the Netherlands., Kemp B; Adaptation Physiology Group, Department of Animal Sciences, Wageningen University and Research, PO Box 338, 6700 AH, Wageningen, the Netherlands., van Hoeij RJ; Adaptation Physiology Group, Department of Animal Sciences, Wageningen University and Research, PO Box 338, 6700 AH, Wageningen, the Netherlands., van Knegsel ATM; Adaptation Physiology Group, Department of Animal Sciences, Wageningen University and Research, PO Box 338, 6700 AH, Wageningen, the Netherlands.. Electronic address: ariette.vanknegsel@wur.nl.
Jazyk:	angličtina
Zdroj:	Journal of dairy science [J Dairy Sci] 2020 May; Vol. 103 (5), pp. 4795-4805. Date of Electronic Publication: 2020 Feb 26.
DOI:	10.3168/jds.2019-17777
Abstrakt:	Negative energy balance in dairy cows in early lactation is related to alteration of metabolic status. However, the relationships among energy balance, metabolic profile in plasma, and metabolic profile in milk have not been reported. In this study our aims were: (1) to reveal the metabolic profiles of plasma and milk by integrating results from nuclear magnetic resonance (NMR) with data from liquid chromatography triple quadrupole mass spectrometry (LC-MS); and (2) to investigate the relationship between energy balance and the metabolic profiles of plasma and milk. For this study 24 individual dairy cows (parity 2.5 ± 0.5; mean ± standard deviation) were studied in lactation wk 2. Body weight (mean ± standard deviation; 627.4 ± 56.4 kg) and milk yield (28.1 ± 6.7 kg/d; mean ± standard deviation) were monitored daily. Milk composition (fat, protein, and lactose) and net energy balance were calculated. Plasma and milk samples were collected and analyzed using LC-MS and NMR. From all plasma metabolites measured, 27 were correlated with energy balance. These plasma metabolites were related to body reserve mobilization from body fat, muscle, and bone; increased blood flow; and gluconeogenesis. From all milk metabolites measured, 30 were correlated with energy balance. These milk metabolites were related to cell apoptosis and cell proliferation. Nine metabolites detected in both plasma and milk were correlated with each other and with energy balance. These metabolites were mainly related to hyperketonemia; β-oxidation of fatty acids; and one-carbon metabolism. The metabolic profiles of plasma and milk provide an in-depth insight into the physiological pathways of dairy cows in negative energy balance in early lactation. In addition to the classical indicators for energy balance (e.g., β-hydroxybutyrate, acetone, and glucose), the current study presents some new metabolites (e.g., glycine in plasma and milk; kynurenine, panthothenate, or arginine in plasma) in lactating dairy cows that are related to energy balance and may be of interest as new indicators for energy balance. (Copyright © 2020 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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