Transcriptome analysis of rice (Oryza sativa L.) in response to ammonium resupply reveals the involvement of phytohormone signaling and the transcription factor OsJAZ9 in reprogramming of nitrogen uptake and metabolism.

Autor: Sun L; State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, No.71 East Beijing Road, Nanjing, Jiangsu, 210008, China; State Key Lab of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University/JCIC-MCP, Nanjing, Jiangsu, 210095, China. Electronic address: sunli@njau.edu.cn., Di DW; State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, No.71 East Beijing Road, Nanjing, Jiangsu, 210008, China. Electronic address: dwdi@issas.ac.cn., Li G; State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, No.71 East Beijing Road, Nanjing, Jiangsu, 210008, China. Electronic address: gjli@issas.ac.cn., Li Y; State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, No.71 East Beijing Road, Nanjing, Jiangsu, 210008, China. Electronic address: ylli@issas.ac.cn., Kronzucker HJ; School of Agriculture and Food, The University of Melbourne, Parkville, VIC 3010, Australia; Faculty of Land and Food Systems, University of British Columbia, Vancouver, BC, V6T 1Z4, Canada. Electronic address: herbert.kronzucker@unimelb.edu.au., Shi W; State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, No.71 East Beijing Road, Nanjing, Jiangsu, 210008, China. Electronic address: wmshi@issas.ac.cn.
Jazyk: angličtina
Zdroj: Journal of plant physiology [J Plant Physiol] 2020 Mar - Apr; Vol. 246-247, pp. 153137. Date of Electronic Publication: 2020 Feb 22.
DOI: 10.1016/j.jplph.2020.153137
Abstrakt: NH 4 + is not only the primary nitrogen for rice, a well-known NH 4 + specialist, but is also the chief limiting factor for its production. Limiting NH 4 + triggers a series of physiological and biochemical responses that help rice optimise its nitrogen acquisition. However, the dynamic nature and spatial distribution of the adjustments at the whole plant level during this response are still unknown. Here, nitrogen-starved rice seedlings were treated with 0.1 mM (NH 4 ) 2 SO 4 for 4 or 12 h, and then the shoots and roots were harvested for RNA-Seq analysis. We identified 138 and 815 differentially expressed genes (DEGs) in shoots, and 597 and 1074 in roots following 4 and 12 h treatment, respectively. Up-regulated DEGs mainly participated in phenylpropanoid, sugar, and amino acid metabolism, which was confirmed by chemical content analysis. The transcription factor OsJAZ9 was the most pronouncedly induced component under low NH 4 + in roots, and a significant increase in root growth, NH 4 + absorption, amino acid, and sugar metabolism in response to resupplied NH 4 + following nitrogen starvation was identified in JAZ9ox (OsJAZ9-overexpressed) and coi1 (OsCOI1-RNAi). Our data provide comprehensive insight into the whole-plant transcriptomic response in terms of metabolic processes and signaling transduction to a low-NH 4 + signal, and identify the transcription factor OsJAZ9 and its involvement in the regulation of carbon/nitrogen metabolism as central to the response to low NH 4 + .
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2020 Elsevier GmbH. All rights reserved.)
Databáze: MEDLINE
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