Presence of The NLRP3 Inflammasome Components in Semen of Varicocele Patients.
| Autor: | Baazm M; Department of Anatomy, School of Medicine, Arak University of Medical Sciences, Arak, Iran. Electronic Address: dr.baazm@arakmu.ac.ir., Ghafarizadeh AA; Infertility Center of ACECR, Arak, Iran., Noshad Kamran AR; Department of Urology, School of Medicine, Arak University of Medical Sciences, Arak, Iran., Beyer C; Institute of Neuroanatomy, RWTH Aachen University, 52074 Aachen, Germany., Zendedel A; Institute of Neuroanatomy, RWTH Aachen University, 52074 Aachen, Germany.; Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. |
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| Jazyk: | angličtina |
| Zdroj: | International journal of fertility & sterility [Int J Fertil Steril] 2020 Apr; Vol. 14 (1), pp. 46-50. Date of Electronic Publication: 2020 Feb 25. |
| DOI: | 10.22074/ijfs.2020.5734 |
| Abstrakt: | Background: Varicocele is a common cause of male infertility with multifactorial etiology. Inflammation is a characteristic pathological event that occurs in the testis tissue following the varicocele. The aim of this study was to investigate expression of nod-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome components and cytokines in semen of varicocele and control subjects. Materials and Methods: In this case-control study, seminal plasma was collected from 32 varicocele patients (with grades 2 and 3) and 20 fertile men as control group. Semen analysis was performed in all subjects. Concentrations of interleukin-1b (IL-1b), IL-18 and caspase-1 in seminal plasma were measured by enzyme-linked immunosorbent assay (ELISA). Apoptosis-associated speck-like protein containing a caspase activation and recruitment domain, in addition to NALP3 were identified in seminal plasma by Western blot. Statistical significance between the mean values was determined by student's t test. Results: According to our data, the level of IL-1b was significantly (P=0.03) increased in the seminal plasma of varicocele patients, compared to the control subjects. We analyzed amount of IL-18 in the both groups. The level of this interleukin was markedly (P=0.002) decreased in varicocele patients. No change was observed in the level of caspase-1 in both groups. Western blot analysis revealed that apoptosis associated speck-like protein (ASC, P=0.0002) and NLRP3 (P=0.005) were significantly elevated in the semen of varicocele patients. Conclusion: This study provides the first evidence of activation of NLRP3 components in semen of men with varicocele. Competing Interests: The authors declare no conflicts of interest. (Copyright© by Royan Institute. All rights reserved.) |
| Databáze: | MEDLINE |
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