Characterization of 100 extended major histocompatibility complex haplotypes in Indonesian cynomolgus macaques.

Autor: Shortreed CG; Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI, 53711, USA., Wiseman RW; Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI, 53711, USA.; Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, WI, 53715, USA., Karl JA; Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI, 53711, USA., Bussan HE; Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI, 53711, USA., Baker DA; Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI, 53711, USA., Prall TM; Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI, 53711, USA., Haj AK; Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI, 53711, USA., Moreno GK; Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI, 53711, USA., Penedo MCT; Veterinary Genetics Laboratory, University of California-Davis, Davis, CA, 95616, USA., O'Connor DH; Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI, 53711, USA. dhoconno@wisc.edu.; Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, WI, 53715, USA. dhoconno@wisc.edu.
Jazyk: angličtina
Zdroj: Immunogenetics [Immunogenetics] 2020 May; Vol. 72 (4), pp. 225-239. Date of Electronic Publication: 2020 Feb 29.
DOI: 10.1007/s00251-020-01159-5
Abstrakt: Many medical advancements-including improvements to anti-rejection therapies in transplantation and vaccine development-rely on preclinical studies conducted in cynomolgus macaques (Macaca fascicularis). Major histocompatibility complex (MHC) class I and class II genes of cynomolgus macaques are orthologous to human leukocyte antigen complex (HLA) class I and class II genes, respectively. Both encode cell-surface proteins involved in cell recognition and rejection of non-host tissues. MHC class I and class II genes are highly polymorphic, so comprehensive genotyping requires the development of complete databases of allelic variants. Our group used PacBio circular consensus sequencing of full-length cDNA amplicons to characterize MHC class I and class II transcript sequences for a cohort of 293 Indonesian cynomolgus macaques (ICM) in a large, pedigreed breeding colony. These studies allowed us to expand the existing database of Macaca fascicularis (Mafa) alleles by identifying an additional 141 MHC class I and 61 class II transcript sequences. In addition, we defined co-segregating combinations of allelic variants as regional haplotypes for 70 Mafa-A, 78 Mafa-B, and 45 Mafa-DRB gene clusters. Finally, we defined class I and class II transcripts that are associated with 100 extended MHC haplotypes in this breeding colony by combining our genotyping analyses with short tandem repeat (STR) patterns across the MHC region. Our sequencing analyses and haplotype definitions improve the utility of these ICM for transplantation studies as well as infectious disease and vaccine research.
Databáze: MEDLINE
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