| Autor: |
Guirado P; Departament de Genètica, Microbiologia i Estadística, Facultat de Biología, Universitat de Barcelona. Avda. Diagonal 643, 08028 Barcelona, Spain., Paytubi S; Departament de Genètica, Microbiologia i Estadística, Facultat de Biología, Universitat de Barcelona. Avda. Diagonal 643, 08028 Barcelona, Spain., Miró E; Hospital de la Santa Creu i Sant Pau and Institut d'Investigació Biomèdica Sant Pau (IIB Sant Pau), Sant Quintí 89, 08041 Barcelona, Spain., Iglesias-Torrens Y; Hospital de la Santa Creu i Sant Pau and Institut d'Investigació Biomèdica Sant Pau (IIB Sant Pau), Sant Quintí 89, 08041 Barcelona, Spain.; Departament de Genètica i Microbiologia. Universitat Autònoma de Barcelona, 08193 Cerdanyola del Vallès (Barcelona), Spain., Navarro F; Hospital de la Santa Creu i Sant Pau and Institut d'Investigació Biomèdica Sant Pau (IIB Sant Pau), Sant Quintí 89, 08041 Barcelona, Spain.; Departament de Genètica i Microbiologia. Universitat Autònoma de Barcelona, 08193 Cerdanyola del Vallès (Barcelona), Spain., Cerdà-Cuéllar M; IRTA, Centre de Recerca en Sanitat Animal (CReSA-IRTA-UAB). Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain., Attolini CS; Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology, Baldiri Reixac 10, 08028 Barcelona, Spain., Balsalobre C; Departament de Genètica, Microbiologia i Estadística, Facultat de Biología, Universitat de Barcelona. Avda. Diagonal 643, 08028 Barcelona, Spain., Madrid C; Departament de Genètica, Microbiologia i Estadística, Facultat de Biología, Universitat de Barcelona. Avda. Diagonal 643, 08028 Barcelona, Spain. |
| Abstrakt: |
Campylobacter jejuni causes campylobacteriosis, a bacterial gastroenteritis with high incidence worldwide. Moreover, C. jejuni infection can trigger the polyneuropathic disorder denominated Guillain-Barré syndrome (GBS). The C. jejuni strains that can elicit GBS carry either wlaN or cgtB, coding both genes for a β-1,3-galactosyltransferase enzyme that is required for the production of sialylated lipooligosaccharide (LOS SIAL ). We described a differential prevalence of the genes wlaN and cgtB in C. jejuni isolates from three different ecological niches: humans, broiler chickens, and wild birds. The distribution of both genes, which is similar between broiler chicken and human isolates and distinct when compared to the wild bird isolates, suggests a host-dependent distribution. Moreover, the prevalence of the wlaN and cgtB genes seems to be restricted to some clonal complexes. Gene sequencing identified the presence of new variants of the G- homopolymeric tract within the wlaN gene. Furthermore, we detected two variants of a G rich region within the cgtB gene, suggesting that, similarly to wlaN , the G-tract in the cgtB gene mediates the phase variation control of cgtB expression. Caco-2 cell invasion assays indicate that there is no evident correlation between the production of LOS SIAL and the ability to invade eukaryotic cells. |
