A helical inner scaffold provides a structural basis for centriole cohesion.

Autor:	Le Guennec M; University of Geneva, Department of Cell Biology, Sciences III, Geneva, Switzerland., Klena N; University of Geneva, Department of Cell Biology, Sciences III, Geneva, Switzerland., Gambarotto D; University of Geneva, Department of Cell Biology, Sciences III, Geneva, Switzerland., Laporte MH; University of Geneva, Department of Cell Biology, Sciences III, Geneva, Switzerland., Tassin AM; Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris Sud, Université Paris-Saclay, 1 Avenue de la Terrasse, 91198 Gif-sur-Yvette, France., van den Hoek H; Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany., Erdmann PS; Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany., Schaffer M; Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany., Kovacik L; Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University of Basel, Basel CH-4058, Switzerland., Borgers S; University of Geneva, Department of Cell Biology, Sciences III, Geneva, Switzerland., Goldie KN; Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University of Basel, Basel CH-4058, Switzerland., Stahlberg H; Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University of Basel, Basel CH-4058, Switzerland., Bornens M; Institut Curie, PSL Research University, CNRS-UMR 144, 75005 Paris, France., Azimzadeh J; Université de Paris, Institut Jacques Monod, CNRS UMR7592, 75013 Paris, France., Engel BD; Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany.; Helmholtz Pioneer Campus, Helmholtz Zentrum München, Ingolstädter Landstraße 1, 85764 Neuherberg, Germany., Hamel V; University of Geneva, Department of Cell Biology, Sciences III, Geneva, Switzerland., Guichard P; University of Geneva, Department of Cell Biology, Sciences III, Geneva, Switzerland.
Jazyk:	angličtina
Zdroj:	Science advances [Sci Adv] 2020 Feb 14; Vol. 6 (7), pp. eaaz4137. Date of Electronic Publication: 2020 Feb 14 (Print Publication: 2020).
DOI:	10.1126/sciadv.aaz4137
Abstrakt:	The ninefold radial arrangement of microtubule triplets (MTTs) is the hallmark of the centriole, a conserved organelle crucial for the formation of centrosomes and cilia. Although strong cohesion between MTTs is critical to resist forces applied by ciliary beating and the mitotic spindle, how the centriole maintains its structural integrity is not known. Using cryo-electron tomography and subtomogram averaging of centrioles from four evolutionarily distant species, we found that MTTs are bound together by a helical inner scaffold covering ~70% of the centriole length that maintains MTTs cohesion under compressive forces. Ultrastructure Expansion Microscopy (U-ExM) indicated that POC5, POC1B, FAM161A, and Centrin-2 localize to the scaffold structure along the inner wall of the centriole MTTs. Moreover, we established that these four proteins interact with each other to form a complex that binds microtubules. Together, our results provide a structural and molecular basis for centriole cohesion and geometry. (Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).)
Databáze:	MEDLINE
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