Fast simultaneous quantification of gabapentin and cetirizine in cell lysates by means of HPLC-MS/MS.

Autor: Schlatt L; University of Münster, Institute of Inorganic and Analytical Chemistry, Corrensstraße 28/30, 48149 Münster, Germany., Costa ACC; University Hospital Münster, Experimental Nephrology, Medicine Clinic D, Albert-Schweitzer-Campus 1/ A14, 48149 Münster, Germany., Barz V; University Hospital Münster, Experimental Nephrology, Medicine Clinic D, Albert-Schweitzer-Campus 1/ A14, 48149 Münster, Germany., Ciarimboli G; University Hospital Münster, Experimental Nephrology, Medicine Clinic D, Albert-Schweitzer-Campus 1/ A14, 48149 Münster, Germany., Karst U; University of Münster, Institute of Inorganic and Analytical Chemistry, Corrensstraße 28/30, 48149 Münster, Germany. Electronic address: uk@uni-muenster.de.
Jazyk: angličtina
Zdroj: Journal of pharmaceutical and biomedical analysis [J Pharm Biomed Anal] 2020 May 30; Vol. 184, pp. 113172. Date of Electronic Publication: 2020 Feb 13.
DOI: 10.1016/j.jpba.2020.113172
Abstrakt: A high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was successfully developed for the simultaneous detection of gabapentin (GBP) and cetirizine (CTZ) in cell lysates. Multiple reaction monitoring (MRM) transitions were found for GBP, CTZ and deuterated internal standards of both, allowing a selective and sensitive detection. Limits of detection (LODs) of 0.04 ng/mL and 0.07 ng/mL were achieved for CTZ and GBP respectively, and a linear range could be confirmed to the highest tested concentration of 50 ng/mL. Furthermore, with a total runtime of 2.5 min, this method allows a high throughput analysis. The applicability of the method was demonstrated by using it for the quantification of CTZ and GBP in cell lysates to examine the effects of renal transporters on these two analytes.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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