Recombinant expression, characterization and phylogenetic studies of novels cystatins-like proteins of sweet orange (Citrus sinensis) and clementine (Citrus clementina).

Autor:	Schneider VK; Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Paulo, Brazil., da Silva Ferrara TF; Departamento de Biofísica, Universidade Federal de São Paulo, São Paulo, Brazil., Rocha SV; Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Paulo, Brazil., Santos-Júnior CD; Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Paulo, Brazil., Neo-Justino DM; Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Paulo, Brazil., da Cunha AF; Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Paulo, Brazil., de Oliveira da Silva JPM; Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Paulo, Brazil., Dos Santos Tersariol IL; Departamento de Bioquímica, Universidade Federal de São Paulo, São Paulo, Brazil., Carmona AK; Departamento de Biofísica, Universidade Federal de São Paulo, São Paulo, Brazil., Henrique-Silva F; Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Paulo, Brazil., Soares-Costa A; Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Paulo, Brazil. Electronic address: andreasc@ufscar.br.
Jazyk:	angličtina
Zdroj:	International journal of biological macromolecules [Int J Biol Macromol] 2020 Jun 01; Vol. 152, pp. 546-553. Date of Electronic Publication: 2020 Feb 25.
DOI:	10.1016/j.ijbiomac.2020.02.280
Abstrakt:	Phytocystatins are plant cystatins that are related to several physiological processes regulating endogenous cysteine proteases involved in seed development and germination, programmed cell death and response to stress conditions. In addition, phytocystatins can act in plant defense against exogenous peptidases from herbivorous insects, pathogens and nematodes. Considering that Citrus fruits are important to human nutrition and represent a high value crop in worldwide agriculture, in the present work, we performed the identification of putative cystatins from Citrus sinensis and from Citrus clementine and submitted them to phylogenetic analysis. Six cystatins from each species were identified as orthologous and classified into three well supported phylogenetic groups. Five cystatins representative of the phylogenetic groups were recombinantly expressed and the in vitro studies revealed them to be potent inhibitors against the cysteine peptidases papain, legumain, human cathepsins (B, L, S, K) and a cathepsin B-like from Diaphorina citri (the Asian Citrus psyllid). Our findings provide the C. clementina and C. sinensis cystatins classification and an enzyme-inhibitor interactions profile, which may reflect an evolutionary process of Citrus cystatins related to gene functions as initial germination rates and seedlings development as well associated to plant defense against pathogens, as insects and nematodes. Competing Interests: Declaration of competing interest The authors declare that there is no conflict of interest in this work. (Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu Full Text from ScienceDirect