Carboxymethyl-kappa-carrageenan: A study of biocompatibility, antioxidant and antibacterial activities.

Autor: Madruga LYC; Institute of Chemistry, Federal University of Rio Grande do Norte (UFRN), Natal, RN, Brazil; Laboratory of Immunoparasitology, College of Pharmacy, Federal University of Rio Grande do Norte (UFRN), Natal, RN, Brazil., Sabino RM; School of Advanced Materials Discovery, Colorado State University, Fort Collins, CO, United States., Santos ECG; Laboratory of Immunoparasitology, College of Pharmacy, Federal University of Rio Grande do Norte (UFRN), Natal, RN, Brazil., Popat KC; School of Advanced Materials Discovery, Colorado State University, Fort Collins, CO, United States; Department of Mechanical Engineering, Colorado State University, Fort Collins, CO, United States; School of Biomedical Engineering, Colorado State University, Fort Collins, CO, United States., Balaban RC; Institute of Chemistry, Federal University of Rio Grande do Norte (UFRN), Natal, RN, Brazil., Kipper MJ; School of Advanced Materials Discovery, Colorado State University, Fort Collins, CO, United States; School of Biomedical Engineering, Colorado State University, Fort Collins, CO, United States; Department of Chemical and Biological Engineering, Colorado State University, Fort Collins, CO, United States. Electronic address: matthew.kipper@colostate.edu.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2020 Jun 01; Vol. 152, pp. 483-491. Date of Electronic Publication: 2020 Feb 25.
DOI: 10.1016/j.ijbiomac.2020.02.274
Abstrakt: Chemical modification of polysaccharides is an important route to enhance, develop or change polysaccharide properties. In this study, carboxymethylation of kappa-carrageenan (KC) with monochloroacetic acid was performed to achieve different degrees of substitution (DS) of carboxymethyl-kappa-carrageenan (CMKC). The degree of substitution ranged from 0.8 to 1.6 and was calculated from the 1 H NMR spectra. The chemical structure of the CMKCs was further characterized by FT-IR, and 13 C NMR. FT-IR confirmed the carboxymethylation. Carboxymethylation increased viscosity of KC in water and decreased viscosity of KC in synthetic human sweat. Tests with human adipose derived stem cells showed higher viability and lower cytotoxicity for CMKCs when compared to KC. CMKCs showed no hemolytic activity to human red blood cells. CMKCs have increased antioxidant activity compared to KC. In antibacterial assays, CMKCs with DS of 0.8, 1.0 and 1.2 exhibited growth inhibition against Staphylococcus aureus, Bacillus cereus, Escherichia coli and Pseudomonas aeruginosa. CMKC with DS ranging from 1.0 to 1.2 are good candidate biomaterials for cell-contacting applications.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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