Upgrading analytical methodology through comparative study for screening of 267 pesticides/metabolites in five representative matrices using UPLC-MS/MS.

Autor: Na TW; National Agricultural Products Quality Management Service (NAQS), 141, Yongjeon-ro, Gimcheon-si, Gyeongsangbuk-do, Republic of Korea., Rahman MM; Natural Products Chemistry Laboratory, Chonnam National University, Yongbong-ro 77, Buk-gu, Gwangju 500-757, Republic of Korea., Kim SW; Jeollanamdo Agricultural Research and Extension Services, Environment-Friendly Agricultural Research Institute, 1508, Senam-ro, Sanpo-myeon, Naju-si, Jeollanamdo 58213, Republic of Korea., Haque ME; Department of Statistics, University of Dhaka, Dhaka 1000, Bangladesh., Eun JB; Department of Food Science and Technology and BK 21 Plus Program, Graduate School of Chonnam National University. Yongbong-ro 77, Buk-gu, Gwangju 500-757, Republic of Korea., Shim JH; Natural Products Chemistry Laboratory, Chonnam National University, Yongbong-ro 77, Buk-gu, Gwangju 500-757, Republic of Korea. Electronic address: jhshim@jnu.ac.kr.
Jazyk: angličtina
Zdroj: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [J Chromatogr B Analyt Technol Biomed Life Sci] 2020 Mar 15; Vol. 1141, pp. 122021. Date of Electronic Publication: 2020 Feb 06.
DOI: 10.1016/j.jchromb.2020.122021
Abstrakt: A comparative study was conducted to replace the traditional screening method (MFDS#83) with the Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) EN method for the determination of 267 pesticides/metabolites/plant activators/growth regulators in five representative crop matrices (mandarin, pepper, potato, rice, and soybean). In the traditional method, samples were extracted with acetonitrile and salt, and purified with a solid-phase extraction cartridge. In the QuEChERS method, the sample extraction was carried out using acetonitrile and a mixture of salts, and purification was performed using dispersive solid phase extraction. The limit of quantification (LOQ) for the MFDS#83 method was 0.0004 mg/kg, whereas for the QuEChERS EN method, the LOQ varied from 0.002 to 0.006 mg/kg for all analytes in various matrices. A six-point matrix-matched calibration curve was prepared for all analytes in five matrices for both methods. Both the MFDS#83 and QuEChERS EN methods provided excellent linearity, with the coefficients of determination (R 2 ) ≥ 0.99 for most of the compounds. In both cases, the method was validated in terms of recovery and repeatability after the fortification of two different concentrations with three replicates for each of the concentrations. The QuEChERS EN method provided better recovery than the MFDS#83 method for all matrices except mandarin.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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