Antimalarial Activity of Meriandra dianthera Leaf Extracts in Plasmodium berghei -Infected Mice.

Autor: Hagazy K; Department of Pharmacy, College of Health Sciences, P.O. Box 298, Aksum University, Axum, Ethiopia., Sibhat GG; Department of Pharmacognosy, School of Pharmacy, College of Health Sciences, Mekelle University, P.O. Box 1871, Mekelle, Ethiopia., Karim A; Department of Pharmacognosy, School of Pharmacy, College of Health Sciences, Mekelle University, P.O. Box 1871, Mekelle, Ethiopia., Tekulu GH; Department of Pharmacognosy, School of Pharmacy, College of Health Sciences, Mekelle University, P.O. Box 1871, Mekelle, Ethiopia., Periasamy G; Department of Pharmacognosy, School of Pharmacy, College of Health Sciences, Mekelle University, P.O. Box 1871, Mekelle, Ethiopia., Hiben MG; Department of Pharmacognosy, School of Pharmacy, College of Health Sciences, Mekelle University, P.O. Box 1871, Mekelle, Ethiopia.
Jazyk: angličtina
Zdroj: Evidence-based complementary and alternative medicine : eCAM [Evid Based Complement Alternat Med] 2020 Feb 12; Vol. 2020, pp. 8980212. Date of Electronic Publication: 2020 Feb 12 (Print Publication: 2020).
DOI: 10.1155/2020/8980212
Abstrakt: Objective: To evaluate the antimalarial effect of aqueous methanolic extract and solvent fractions of Meriandra dianthera leaves against Plasmodium berghei in mice model.
Method: M. dianthera leaves were extracted with 80% methanol and dried. The dried crude extract was then defatted and further fractionated with chloroform, ethyl acetate, and butanol. Acute oral toxicity test was performed as per the Organization for Economic Cooperation and Development guideline 425. Peter's 4-day suppressive test was used to determine the in vivo antimalarial activity of the extract and fractions.
Result: The crude leaf extract of Meriandra dianthera leaves against P < 0.001) chemosuppression compared to the negative control. Both the extract and fractions were able to prevent P. berghei induced body weight loss and body temperature reduction and also increased the survival time of the mice as compared to the negative control. The aqueous methanolic leaf extract of M. dianthera leaves were extracted with 80% methanol and dried. The dried crude extract was then defatted and further fractionated with chloroform, ethyl acetate, and butanol. Acute oral toxicity test was performed as per the Organization for Economic Cooperation and Development guideline 425. Peter's 4-day suppressive test was used to determine the.
Conclusion: The extracts of M. dianthera leaves showed promising antimalarial activity, with no sign of toxicity and therefore may support its traditional use for the treatment of malaria. M. dianthera leaves were extracted with 80% methanol and dried. The dried crude extract was then defatted and further fractionated with chloroform, ethyl acetate, and butanol. Acute oral toxicity test was performed as per the Organization for Economic Cooperation and Development guideline 425. Peter's 4-day suppressive test was used to determine the.
Competing Interests: The authors declare that there are no conflicts of interest.
(Copyright © 2020 Kalay Hagazy et al.)
Databáze: MEDLINE
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