Attenuated Total Reflection-Fourier Transform Infrared (ATR-FTIR) Spectroscopy Analysis of Saliva for Breast Cancer Diagnosis.

Autor: Ferreira ICC; Laboratory of Nanobiotechnology, Institute of Biotechnology, Federal University of Uberlandia, Uberlandia 38405-302, Brazil., Aguiar EMG; Department of Physiology, Institute of Biomedical Sciences, Federal University of Uberlandia, Uberlandia 38405-302, Brazil., Silva ATF; Laboratory of Nanobiotechnology, Institute of Biotechnology, Federal University of Uberlandia, Uberlandia 38405-302, Brazil., Santos LLD; Laboratory of Nanobiotechnology, Institute of Biotechnology, Federal University of Uberlandia, Uberlandia 38405-302, Brazil., Cardoso-Sousa L; Department of Physiology, Institute of Biomedical Sciences, Federal University of Uberlandia, Uberlandia 38405-302, Brazil., Araújo TG; Laboratory of Nanobiotechnology, Institute of Biotechnology, Federal University of Uberlandia, Uberlandia 38405-302, Brazil., Santos DW; Obstetric Division, University Hospital, Federal University of Uberlandia, Uberlandia 38405-320, Brazil., Goulart LR; Laboratory of Nanobiotechnology, Institute of Biotechnology, Federal University of Uberlandia, Uberlandia 38405-302, Brazil., Sabino-Silva R; Department of Physiology, Institute of Biomedical Sciences, Federal University of Uberlandia, Uberlandia 38405-302, Brazil., Maia YCP; Laboratory of Nanobiotechnology, Institute of Biotechnology, Federal University of Uberlandia, Uberlandia 38405-302, Brazil.; School of Medicine, Federal University of Uberlandia, Uberlandia 38405-320, Brazil.
Jazyk: angličtina
Zdroj: Journal of oncology [J Oncol] 2020 Feb 10; Vol. 2020, pp. 4343590. Date of Electronic Publication: 2020 Feb 10 (Print Publication: 2020).
DOI: 10.1155/2020/4343590
Abstrakt: Saliva biomarkers using reagent-free biophotonic technology have not been investigated as a strategy for early detection of breast cancer (BC). The attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy has been proposed as a promising tool for disease diagnosis. However, its utilization in cancer is still incipient, and currently saliva has not been used for BC screening. We have applied ATR-FTIR onto saliva from patients with breast cancer, benign breast disease, and healthy matched controls to investigate its potential use in BC diagnosis. Several salivary vibrational modes have been identified in original and second-derivative spectra. The absorbance levels at wavenumber 1041 cm -1 were significantly higher ( p < 0.05) in saliva of breast cancer patients compared with those of benign patients, and the ROC curve analysis of this peak showed a reasonable accuracy to discriminate breast cancer from benign and control patients. The 1433-1302.9 cm -1 band area was significantly higher ( p < 0.05) in saliva of breast cancer patients than in control and benign patients. This salivary ATR-FTIR spectral area was prevalidated as a potential diagnostic biomarker of BC. This spectral biomarker was able to discriminate human BC from controls with sensitivity and specificity of 90% and 80%, respectively. Besides, it was able to differentiate BC from benign disease with sensitivity and specificity of 90% and 70%, respectively. Briefly, for the first time, saliva analysis by ATR-FTIR spectroscopy has demonstrated the potential use of salivary spectral biomarkers (1041 cm -1 and 1433-1302.9 cm -1 ) as a novel alternative for noninvasive BC diagnosis, which could be used for screening purposes.
Competing Interests: There are no conflicts to declare.
(Copyright © 2020 Izabella C. C. Ferreira et al.)
Databáze: MEDLINE
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