Synthesis of Silver Nanoparticle Employing Corn Cob Xylan as a Reducing Agent with Anti- Trypanosoma cruzi Activity.

Autor:	Brito TK; Postgraduate Program in Health Sciences, Federal University of Rio Grande do Norte (UFRN), Natal, Rio Grande do Norte 59012-570, Brazil.; Laboratory of Biotechnology of Natural Polymers (BIOPOL), Department of Biochemistry, Center of Biosciences, Federal University of Rio Grande do Norte (UFRN), Natal, Rio Grande do Norte 59078-970, Brazil., Silva Viana RL; Laboratory of Biotechnology of Natural Polymers (BIOPOL), Department of Biochemistry, Center of Biosciences, Federal University of Rio Grande do Norte (UFRN), Natal, Rio Grande do Norte 59078-970, Brazil.; Postgraduate Program in Biochemistry, Federal University of Rio Grande do Norte UFRN), Natal, Rio Grande do Norte 59078-970, Brazil., Gonçalves Moreno CJ; Postgraduate Program in Biochemistry, Federal University of Rio Grande do Norte UFRN), Natal, Rio Grande do Norte 59078-970, Brazil.; Laboratory of Immunoparasitology, Department of Clinical and Toxicological Analysis, Health Sciences Center, Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte 59012-570, Brazil., da Silva Barbosa J; Postgraduate Program in Health Sciences, Federal University of Rio Grande do Norte (UFRN), Natal, Rio Grande do Norte 59012-570, Brazil.; Laboratory of Biotechnology of Natural Polymers (BIOPOL), Department of Biochemistry, Center of Biosciences, Federal University of Rio Grande do Norte (UFRN), Natal, Rio Grande do Norte 59078-970, Brazil.; Federal Institute of Education, Science and Technology of Rio Grande do Norte (IFRN), Natal, Rio Grande do Norte 59500-000, Brazil., Lopes de Sousa Júnior F; Laboratory of Chemistry of Coordination and Polymers (LQCPol), Institute of Chemistry, Federal University of Rio Grande do Norte (UFRN), Natal, Rio Grande do Norte 59078-970, Brazil., Campos de Medeiros MJ; Laboratory of Chemistry of Coordination and Polymers (LQCPol), Institute of Chemistry, Federal University of Rio Grande do Norte (UFRN), Natal, Rio Grande do Norte 59078-970, Brazil., Melo-Silveira RF; Laboratory of Biotechnology of Natural Polymers (BIOPOL), Department of Biochemistry, Center of Biosciences, Federal University of Rio Grande do Norte (UFRN), Natal, Rio Grande do Norte 59078-970, Brazil.; Postgraduate Program in Biochemistry, Federal University of Rio Grande do Norte UFRN), Natal, Rio Grande do Norte 59078-970, Brazil., Almeida-Lima J; Postgraduate Program in Health Sciences, Federal University of Rio Grande do Norte (UFRN), Natal, Rio Grande do Norte 59012-570, Brazil.; Laboratory of Biotechnology of Natural Polymers (BIOPOL), Department of Biochemistry, Center of Biosciences, Federal University of Rio Grande do Norte (UFRN), Natal, Rio Grande do Norte 59078-970, Brazil.; Postgraduate Program in Biochemistry, Federal University of Rio Grande do Norte UFRN), Natal, Rio Grande do Norte 59078-970, Brazil., de Lima Pontes D; Laboratory of Chemistry of Coordination and Polymers (LQCPol), Institute of Chemistry, Federal University of Rio Grande do Norte (UFRN), Natal, Rio Grande do Norte 59078-970, Brazil., Sousa Silva M; Postgraduate Program in Biochemistry, Federal University of Rio Grande do Norte UFRN), Natal, Rio Grande do Norte 59078-970, Brazil.; Laboratory of Immunoparasitology, Department of Clinical and Toxicological Analysis, Health Sciences Center, Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte 59012-570, Brazil.; Global Health and Tropical Medicine, Institute of Hygiene and Tropical Medicine, New University of Lisbon, Lisboa 1349-008, Portugal., Oliveira Rocha HA; Postgraduate Program in Health Sciences, Federal University of Rio Grande do Norte (UFRN), Natal, Rio Grande do Norte 59012-570, Brazil.; Laboratory of Biotechnology of Natural Polymers (BIOPOL), Department of Biochemistry, Center of Biosciences, Federal University of Rio Grande do Norte (UFRN), Natal, Rio Grande do Norte 59078-970, Brazil.; Postgraduate Program in Biochemistry, Federal University of Rio Grande do Norte UFRN), Natal, Rio Grande do Norte 59078-970, Brazil.
Jazyk:	angličtina
Zdroj:	International journal of nanomedicine [Int J Nanomedicine] 2020 Feb 12; Vol. 15, pp. 965-979. Date of Electronic Publication: 2020 Feb 12 (Print Publication: 2020).
DOI:	10.2147/IJN.S216386
Abstrakt:	Background: Chagas disease, also known as American Trypanosomiasis, is caused by the protozoan Trypanosoma cruzi . It is occurring in Americas, including USA and Canada, and Europe and its current treatment involves the use of two drugs as follows: benznidazole (BNZ) and nifurtimox, which present high toxicity and low efficacy during the chronic phase of the disease, thus promoting the search for more effective therapeutic alternatives. Amongst them xylan, a bioactive polysaccharide, extracted from corn cob. Methods: Ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy (FITR), Raman spectroscopy, energy-dispersive X-ray spectroscopy (EDS), scanning electron microscopy, atomic force microscopy, plasma optical emission spectroscopy (ICP-OES), dynamic light scattering (DLS) have been used to characterize the silver-xylan nanoparticles (NX). Their cytotoxicity was evaluated with 3-bromo(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) test. MTT and flow cytometry were used to ascertain the anti- Trypanosoma cruzi activity. Results: UV-Vis spectroscopy gave plasmon resonance ranging between 400 and 450 nm while FITC and Raman spectroscopy proved nano interface functionalized with xylan. ICP-OES data showed NX with xylan (81%) and silver (19%). EDS showed NX consisting of carbon (59.4%), oxygen (26.2%) and silver (4.8%) main elements. Spherical NX of 55 nm average size has been depicted with SEM and AFM, while DLS showed 102 ± 1.7 nm NX. The NX displayed negligible cytotoxicity (2000 µg/mL). NX (100 µg/mL) was more effective, regardless of experiment time, in affecting the ability of parasites to reduce MTT than BZN (100 µg/mL). In addition, NX (100 µg/mL) induced death of 95% of parasites by necrosis. Conclusion: This is the first time silver nanoparticles are presented as an anti- Trypanosoma cruzi agent and the data point to the potential application of NX to new preclinical studies in vitro and in vivo. Competing Interests: Rony Viana and Mayara Medeiros received a MSc. scholarship from CAPES, and Claudia Moreno had a Ph.D. scholarship from CAPES. This research was submitted to the Graduate Program in Ciências da Saúde (Health Sciences – loose translation) at UFRN, as part of the doctoral thesis of Talita Katiane de Brito. The authors report no other conflicts of interest in this work. (© 2020 Brito et al.)
Databáze:	MEDLINE
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