Regulation of Proteins in Human Skeletal Muscle: The Role of Transcription.

Autor:	Makhnovskii PA; Institute of Biomedical Problems of the Russian Academy of Sciences, Moscow, 123007, Russia., Zgoda VG; V.N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, 119121, Russia., Bokov RO; Institute of Biomedical Problems of the Russian Academy of Sciences, Moscow, 123007, Russia., Shagimardanova EI; Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, Kazan, 420012, Russia., Gazizova GR; Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, Kazan, 420012, Russia., Gusev OA; Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, Kazan, 420012, Russia.; KFU-RIKEN Translational Genomics Unit, Cluster for Science, Technology and Innovation Hub, RIKEN, Saitama, 351-0198, Japan.; RIKEN Preventive Medicine & Diagnosis Innovation Program, RIKEN, Yokohama, 230-0045, Japan.; RIKEN Center for Integrative Medical Sciences, RIKEN, Yokohama, 230-0045, Japan., Lysenko EA; Institute of Biomedical Problems of the Russian Academy of Sciences, Moscow, 123007, Russia.; Faculty of Fundamental Medicine, M.V. Lomonosov Moscow State University, Moscow, 119991, Russia., Kolpakov FA; Institute of Computational Technologies of the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia., Vinogradova OL; Institute of Biomedical Problems of the Russian Academy of Sciences, Moscow, 123007, Russia.; Faculty of Fundamental Medicine, M.V. Lomonosov Moscow State University, Moscow, 119991, Russia., Popov DV; Institute of Biomedical Problems of the Russian Academy of Sciences, Moscow, 123007, Russia. danil-popov@yandex.ru.; Faculty of Fundamental Medicine, M.V. Lomonosov Moscow State University, Moscow, 119991, Russia. danil-popov@yandex.ru.
Jazyk:	angličtina
Zdroj:	Scientific reports [Sci Rep] 2020 Feb 26; Vol. 10 (1), pp. 3514. Date of Electronic Publication: 2020 Feb 26.
DOI:	10.1038/s41598-020-60578-2
Abstrakt:	Regular low intensity aerobic exercise (aerobic training) provides effective protection against various metabolic disorders. Here, the roles played by transient transcriptome responses to acute exercise and by changes in baseline gene expression during up-regulation of protein content in human skeletal muscle were investigated after 2 months of aerobic training. Seven untrained males were involved in a 2 month aerobic cycling training program. Mass-spectrometry and RNA sequencing were used to evaluate proteome and transcriptome responses to training and acute exercise. We found that proteins with different functions are regulated differently at the transcriptional level; for example, a training-induced increase in the content of extracellular matrix-related proteins is regulated at the transcriptional level, while an increase in the content of mitochondrial proteins is not. An increase in the skeletal muscle content of several proteins (including mitochondrial proteins) was associated with increased protein stability, which is related to a chaperone-dependent mechanism and/or reduced regulation by proteolysis. These findings increase our understanding of the molecular mechanisms underlying regulation of protein expression in human skeletal muscle subjected to repeated stress (long term aerobic training) and may provide an opportunity to control the expression of specific proteins (e.g., extracellular matrix-related proteins, mitochondrial proteins) through physiological and/or pharmacological approaches.
Databáze:	MEDLINE
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