Accumulation of CD4 and CD8 T Cells in Placenta of Malaria Infected Mice Induces the Expression of Hypoxia Inducible Factor-1α (HIF-1α) and Low Birth Weight (LBW) of the Fetus.

Autor:	Rahmah Z; Laboratory of Parasitology, Faculty of Medicine and Health Sciences, Universitas Islam Negeri Maulana Malik Ibrahim, Malang, Indonesia., Wahju-Sardjono T; Department of Parasitology, Malaria Research Group, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia., Enggar-Fitri L; Department of Parasitology, Malaria Research Group, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia., Ulfiati A; Master Program in Biomedical Science, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia., Ungu B; Medicine Study Program, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia., Zulhaidah-Arthamin M; Department of Clinical Pathology, Dr. Saiful Anwar General Hospital, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia., Norahmawati E; Department of Pathology, Dr. Saiful Anwar General Hospital, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia.
Jazyk:	angličtina
Zdroj:	Iranian journal of parasitology [Iran J Parasitol] 2019 Oct-Dec; Vol. 14 (4), pp. 604-613.
Abstrakt:	Background: Placental malaria involves the sequestration of infected erythrocytes and infiltration of monocytes, helper T cells (CD4), cytotoxic T cells (CD8) as well as T-cell intracellular antigen-1 (TIA-1) in placental intervillous space. These may interferes the nutrient and oxygen transport, causing placental hypoxia and insufficiency that may affect the fetal growth. This study aimed to prove whether the infiltration of lymphocytes in placental malaria mice increases the expression of HIF-1α thus causes fetal Low Birth Weight (LBW). Methods: Nine pregnant BALB/c mice that infected with Plasmodium berghei ANKA strain on day 9 post mating were used as treatment group and 8 non infected pregnant mice were used as control group. The mice were sacrificed on day 18 post mating; then the fetus was weighed individually and the placentas were isolated separately. Expression of CD4, CD8 and HIF-1α were counted by immunohistochemistry using CD4 monoclonal Ab (Santa cruz, sc-59031 CD4) and CD 8 monoclonal Ab (NeoMarker RM-9116-S0) as well as anti-HIF-1α antibody (H1α67) ChIP Grade from Abcam. Results: There was a higher expression of CD8, CD4 and HIF-1α in infected placenta compare to normal placenta. Analysis using Structural Equation Modeling (SEM) showed expression CD8 and CD4 caused an increase expression of HIF-1α in placenta (t ≥1.96). Expression of HIF-1α caused low fetal weight (t ≥1.96). Conclusion: In placental malaria, the expression of CD4 and CD8 induce placental hypoxia characterized by increased expression of HIF-1α that causes LBW. Competing Interests: Conflict of interests None of the authors has any conflict of interest in regard to this article (Copyright© Iranian Society of Parasitology & Tehran University of Medical Sciences.)
Databáze:	MEDLINE
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