Mechanical properties measured by atomic force microscopy define health biomarkers in ageing C. elegans.

Autor:	Essmann CL; Department of Computer Science, University College London, Engineering Building, Malet Place, London, WC1E 7JG, UK. c.essmann@ucl.ac.uk.; Institute of Structural and Molecular Biology, University College London and Birkbeck, London, WC1E 6BT, UK. c.essmann@ucl.ac.uk.; MRC London Institute of Medical Sciences, Du Cane Road, London, W12 0NN, UK. c.essmann@ucl.ac.uk.; Institute of Clinical Sciences, Imperial College London, Hammersmith Hospital Campus, Du Cane Road, London, W12 0NN, UK. c.essmann@ucl.ac.uk., Martinez-Martinez D; MRC London Institute of Medical Sciences, Du Cane Road, London, W12 0NN, UK.; Institute of Clinical Sciences, Imperial College London, Hammersmith Hospital Campus, Du Cane Road, London, W12 0NN, UK., Pryor R; Institute of Structural and Molecular Biology, University College London and Birkbeck, London, WC1E 6BT, UK.; MRC London Institute of Medical Sciences, Du Cane Road, London, W12 0NN, UK.; Institute of Clinical Sciences, Imperial College London, Hammersmith Hospital Campus, Du Cane Road, London, W12 0NN, UK., Leung KY; UCL Great Ormond Street Institute of Child Health, University College London, London, WC1N 1EH, UK., Krishnan KB; Institute of Structural and Molecular Biology, University College London and Birkbeck, London, WC1E 6BT, UK., Lui PP; Institute of Structural and Molecular Biology, University College London and Birkbeck, London, WC1E 6BT, UK., Greene NDE; UCL Great Ormond Street Institute of Child Health, University College London, London, WC1N 1EH, UK., Brown AEX; MRC London Institute of Medical Sciences, Du Cane Road, London, W12 0NN, UK.; Institute of Clinical Sciences, Imperial College London, Hammersmith Hospital Campus, Du Cane Road, London, W12 0NN, UK., Pawar VM; Department of Computer Science, University College London, Engineering Building, Malet Place, London, WC1E 7JG, UK., Srinivasan MA; Department of Computer Science, University College London, Engineering Building, Malet Place, London, WC1E 7JG, UK.; Department of Mechanical Engineering and Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA., Cabreiro F; Institute of Structural and Molecular Biology, University College London and Birkbeck, London, WC1E 6BT, UK. f.cabreiro@lms.mrc.ac.uk.; MRC London Institute of Medical Sciences, Du Cane Road, London, W12 0NN, UK. f.cabreiro@lms.mrc.ac.uk.; Institute of Clinical Sciences, Imperial College London, Hammersmith Hospital Campus, Du Cane Road, London, W12 0NN, UK. f.cabreiro@lms.mrc.ac.uk.
Jazyk:	angličtina
Zdroj:	Nature communications [Nat Commun] 2020 Feb 25; Vol. 11 (1), pp. 1043. Date of Electronic Publication: 2020 Feb 25.
DOI:	10.1038/s41467-020-14785-0
Abstrakt:	Genetic and environmental factors are key drivers regulating organismal lifespan but how these impact healthspan is less well understood. Techniques capturing biomechanical properties of tissues on a nano-scale level are providing new insights into disease mechanisms. Here, we apply Atomic Force Microscopy (AFM) to quantitatively measure the change in biomechanical properties associated with ageing Caenorhabditis elegans in addition to capturing high-resolution topographical images of cuticle senescence. We show that distinct dietary restriction regimes and genetic pathways that increase lifespan lead to radically different healthspan outcomes. Hence, our data support the view that prolonged lifespan does not always coincide with extended healthspan. Importantly, we identify the insulin signalling pathway in C. elegans and interventions altering bacterial physiology as increasing both lifespan and healthspan. Overall, AFM provides a highly sensitive technique to measure organismal biomechanical fitness and delivers an approach to screen for health-improving conditions, an essential step towards healthy ageing.
Databáze:	MEDLINE
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