Characterization and molecular epidemiology of Staphylococcus aureus strains resistant to beta-lactams isolated from the milk of cows diagnosed with subclinical mastitis.
| Autor: | Souza GÁAD; Federal University of Minas Gerais, Institute of Agrarian Sciences, Center for Research in Agrarian Sciences, Laboratory of Animal Health, Montes Claros, Minas Gerais, Brazil., de Almeida AC; Federal University of Minas Gerais, Institute of Agrarian Sciences, Center for Research in Agrarian Sciences, Laboratory of Animal Health, Montes Claros, Minas Gerais, Brazil., Xavier MAS; State University of Montes Claros, Center of Biological and Health Sciences, Microbiology Laboratory, Montes Claros, Minas Gerais, Brazil., da Silva LMV; Federal University of Minas Gerais, Institute of Agrarian Sciences, Center for Research in Agrarian Sciences, Laboratory of Animal Health, Montes Claros, Minas Gerais, Brazil., Sousa CN; Federal University of Minas Gerais, Institute of Agrarian Sciences, Center for Research in Agrarian Sciences, Laboratory of Animal Health, Montes Claros, Minas Gerais, Brazil., Sanglard DA; Federal University of Minas Gerais, Institute of Agrarian Sciences, Center for Research in Agrarian Sciences, Laboratory of Biotechnology, Montes Claros, Minas Gerais, Brazil., Xavier AREO; Federal University of Minas Gerais, Institute of Agrarian Sciences, Center for Research in Agrarian Sciences, Laboratory of Animal Health, Montes Claros, Minas Gerais, Brazil. |
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| Jazyk: | angličtina |
| Zdroj: | Veterinary world [Vet World] 2019 Dec; Vol. 12 (12), pp. 1931-1939. Date of Electronic Publication: 2019 Dec 11. |
| DOI: | 10.14202/vetworld.2019.1931-1939 |
| Abstrakt: | Background and Aim: The term ESKAPE, recognized by the WHO, is an acronym, which refers to the pathogens Enterococcus faecium , Staphylococcus aureus , Klebsiella pneumoniae , Acinetobacter baumannii , Pseudomonas aeruginosa , and Enterobacter spp., which is extremely virulent and multidrug-resistant. Although the term is used to designate nosocomial pathogens, in a milking environment, strains of Methicillin-resistant S. aureus have been isolated from cattle diagnosed with clinical and subclinical mastitis. Resistant strains may be involved in the transfer of genes conferring resistance to beta-lactam antimicrobials among the species of microorganisms related to mastitis etiology. This study aimed to trace the phenotypic and genotypic profiles of susceptibility to beta-lactams in S. aureus isolated from milk of cattle diagnosed with subclinical mastitis obtained from different rural properties located in the North of Minas Gerais State, Brazil. Materials and Methods: Sixteen microorganisms previously identified as S. aureus isolated from milk of cattle diagnosed with subclinical mastitis were submitted to matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF), mass spectrometry, and polymerase chain reaction (PCR) analysis for microbial species confirmation. The S. aureus beta-lactams antimicrobial phenotypic resistance profile was investigated by disk diffusion method. PCR methods were also performed to investigate the S. aureus genotypic beta-lactams resistance profile. For this purpose, bla Results: All isolates showed phenotypic resistance to at least three beta-lactams, among which was meropenem. None of the isolates tested positive for the genes mec Conclusion: In this study, we identified beta-lactams resistant S. aureus strains isolated from the milk of cows diagnosed with subclinical mastitis. The S. aureus beta-lactams resistance was investigated using a phenotypic and genotypic approach. We believe that molecular epidemiology, improved knowledge, and genetic basis of resistance to beta-lactams might assist in asserting guidelines for better management practices of dealing with subclinical mastitis and mapping of origin of resistant pathogens in the studied Brazilian area. (Copyright: © Souza, et al.) |
| Databáze: | MEDLINE |
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