Agrobacterium-mediated transformation of arthroconidia obtained from the edible mushroom Hypsizygus marmoreus.

Autor: Bao D; College of Food Science, Shanghai Ocean University, 999 Huchenghuan Road, Pudong District, Shanghai 201306, China; Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, 1000 Jingqi road, Fengxian District, Shanghai 201403, China., Huang Z; College of Food Science, Shanghai Ocean University, 999 Huchenghuan Road, Pudong District, Shanghai 201306, China., Li Y; Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, 1000 Jingqi road, Fengxian District, Shanghai 201403, China., Zhou C; Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, 1000 Jingqi road, Fengxian District, Shanghai 201403, China., Li Y; Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, 1000 Jingqi road, Fengxian District, Shanghai 201403, China., Wan J; Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, 1000 Jingqi road, Fengxian District, Shanghai 201403, China., Tang L; Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, 1000 Jingqi road, Fengxian District, Shanghai 201403, China., Mao W; Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, 1000 Jingqi road, Fengxian District, Shanghai 201403, China., Wang Y; Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, 1000 Jingqi road, Fengxian District, Shanghai 201403, China., Gong M; Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, 1000 Jingqi road, Fengxian District, Shanghai 201403, China., Zou G; Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, 1000 Jingqi road, Fengxian District, Shanghai 201403, China., Honda Y; Laboratory of Forest Biochemistry, Division of Environmental Science and Technology, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwake-cho, Sakyoku-ku, Kyoto 606-8502, Japan., Yang R; Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, 1000 Jingqi road, Fengxian District, Shanghai 201403, China. Electronic address: yangruiheng@126.com., Shang J; College of Food Science, Shanghai Ocean University, 999 Huchenghuan Road, Pudong District, Shanghai 201306, China; Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, 1000 Jingqi road, Fengxian District, Shanghai 201403, China. Electronic address: shangjunjun@saas.sh.cn.
Jazyk: angličtina
Zdroj: Journal of microbiological methods [J Microbiol Methods] 2020 Apr; Vol. 171, pp. 105878. Date of Electronic Publication: 2020 Feb 21.
DOI: 10.1016/j.mimet.2020.105878
Abstrakt: Using the carboxin resistance gene from Pleurotus eryngii as a selective marker, we introduced foreign DNA into the arthroconidia of Hypsizygus marmoreus through Agrobacterium-mediated transformation. The function of the exogenous GUS (β-glucuronidase) gene driven by the CaMV35S promoter was detected in the transformants.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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