Premature chromosome condensation assay to study influence of high-level natural radiation on the initial DNA double strand break repair in human G 0 lymphocytes.

Autor: Vivek Kumar PR; Low Level Radiation Research Laboratory, Low Level Radiation Research Section (LLRRS), Radiation Biology & Health Sciences Division (RB&HSD), Bio-Science Group, Bhabha Atomic Research Centre (BARC), Beach Road, Kollam, 691 001, Kerala, India. Electronic address: prvkumar@barc.gov.in., Karuppasamy CV; Low Level Radiation Research Laboratory, Low Level Radiation Research Section (LLRRS), Radiation Biology & Health Sciences Division (RB&HSD), Bio-Science Group, Bhabha Atomic Research Centre (BARC), Beach Road, Kollam, 691 001, Kerala, India., Ramachandran EN; Low Level Radiation Research Laboratory, Low Level Radiation Research Section (LLRRS), Radiation Biology & Health Sciences Division (RB&HSD), Bio-Science Group, Bhabha Atomic Research Centre (BARC), Beach Road, Kollam, 691 001, Kerala, India., Anil Kumar V; Low Level Radiation Research Laboratory, Low Level Radiation Research Section (LLRRS), Radiation Biology & Health Sciences Division (RB&HSD), Bio-Science Group, Bhabha Atomic Research Centre (BARC), Beach Road, Kollam, 691 001, Kerala, India., Jaikrishan G; Low Level Radiation Research Laboratory, Low Level Radiation Research Section (LLRRS), Radiation Biology & Health Sciences Division (RB&HSD), Bio-Science Group, Bhabha Atomic Research Centre (BARC), Beach Road, Kollam, 691 001, Kerala, India., Das B; LLRRS, RB&HSD, BARC, Trombay, Mumbai, 400 085, India. Electronic address: biraj@barc.gov.in.
Jazyk: angličtina
Zdroj: Mutation research. Genetic toxicology and environmental mutagenesis [Mutat Res Genet Toxicol Environ Mutagen] 2020 Jan; Vol. 849, pp. 503141. Date of Electronic Publication: 2020 Jan 18.
DOI: 10.1016/j.mrgentox.2020.503141
Abstrakt: The inherent capacity of individuals to efficiently repair ionizing radiation induced DNA double strand breaks (DSBs) may be inherited, however, it is influenced by several epigenetic and environmental factors. A pilot study tested whether chronic low dose natural radiation exposure influences the rejoining of initial DNA DSBs induced by a 2 Gy γ-irradiation in 22 individuals from high (>1.5 mGy/year) and normal (≤1.5 mGy/year) level natural radiation areas (H&NLNRA) of Kerala. Rejoining of DSBs (during 1 h at 37 °C, immediately after irradiation) was evaluated at the chromosome level in the presence and absence of wortmannin (a potent inhibitor of DSB repair in normal human cells) using a cell fusion-induced premature chromosome condensation (PCC) assay. The PCC assay quantitates DSBs in the form of excess chromosome fragments in human G 0 lymphocytes without the requirement for cell division. A quantitative difference was observed in the early rejoining of DNA DSBs between individuals from HLNRA and NLNRA, with HLNRA individuals showing a higher (P = 0.05) mean initial repair ratio. The results indicate an influence of chronic low dose natural radiation on initial DNA DSB repair in inhabitants of HLNRA of the Kerala coast.
Competing Interests: Declaration of Competing Interest The authors declare that there are no conflicts of interest.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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