Canine umbilical cord perivascular tissue: A source of stem cells for therapy and research.

Autor: Sepúlveda RV; Veterinary Department, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil., Eleotério In Memorian RB; Veterinary Department, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil., Valente FL; Veterinary Department, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil., Araújo FR; Veterinary Department, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil., Sabino AP; Department of Clinical and Toxicological Analysis, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Brazil., Evangelista FCG; Department of Clinical and Toxicological Analysis, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Brazil., Reis ECC; Veterinary Department, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil. Electronic address: emily.carlo@ufv.br., Borges APB; Veterinary Department, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil. Electronic address: andrea@ufv.br.
Jazyk: angličtina
Zdroj: Research in veterinary science [Res Vet Sci] 2020 Apr; Vol. 129, pp. 193-202. Date of Electronic Publication: 2020 Feb 13.
DOI: 10.1016/j.rvsc.2020.02.006
Abstrakt: There are numerous sources of multipotent mesenchymal stromal cells (MSC) with therapeutic potential, and bone marrow is the main one. However, pain, lack of donors and comorbidities associated with harvesting stimulate the search for new sources of MSCs. The aim of this work is to obtain cells from umbilical cord (UC) perivascular tissue of dogs and characterize them as MSCs. For this, the UC was obtained from therapeutic cesarean sections and submitted to enzymatic digestion. The obtained cells were subjected to growth and proliferation tests, as well as the analysis of surface markers, differentiation test in three mesenchymal lineages and analysis of differentiation markers expression. From all the UC used in this study an adherent with fibroblastoid shape cell was obtained, with an initial number of 4.8 × 10 5 of cells. The growth curves showed a lag phase from 0 to 24 h, followed by a phase of growth of 24 to 168 h, and then phase of cell decay. The doubling time was kept around 15 h until the sixth passage, from which there were signs of cellular senescence. The differentiation assays demonstrated the ability of cells to differentiate into osteoblasts, adipocytes and chondrocytes when subjected to the induction mediums. The study of surface markers was positive for adhesion markers and negative for hematopoietic markers. Thus, cells obtained from canine UC perivascular tissue by enzymatic digestion are multipotent MSC and the protocol developed ensures the perivascular origin of these cells.
Competing Interests: Declaration of Competing Interest The authors declare no conflict of interest.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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