Re-track: Software to analyze the retraction and protrusion velocities of neurites, filopodia and other structures.

Autor: Mousavi SI; Department of Physics, Worcester Polytechnic Institute, 100 Institute Road, Worcester, MA, 01609, USA., Pearce KM; Department of Chemistry and Biochemistry, Worcester Polytechnic Institute, 100 Institute Road, Worcester, MA, 01609, USA., Scarlata S; Department of Chemistry and Biochemistry, Worcester Polytechnic Institute, 100 Institute Road, Worcester, MA, 01609, USA. Electronic address: sfscarlata@wpi.edu., Tüzel E; Department of Physics, Worcester Polytechnic Institute, 100 Institute Road, Worcester, MA, 01609, USA. Electronic address: erkan.tuzel@temple.edu.
Jazyk: angličtina
Zdroj: Analytical biochemistry [Anal Biochem] 2020 May 01; Vol. 596, pp. 113626. Date of Electronic Publication: 2020 Feb 17.
DOI: 10.1016/j.ab.2020.113626
Abstrakt: We have developed new software, Re-track, that will quantify the rates of retraction and protrusion of structures emanating from the central core of a cell, such as neurites or filopodia. Re-Track, uses time-lapse images of cells in TIFF format and calculates the velocity of retraction or protrusion of a selected structure. The software uses a flexible moving boundary and has the ability to correct this boundary throughout analysis. Re-Track is fast, platform independent, and user friendly, and it can be used to follow biological events such as changes in neuronal connections, tip-growing cells such as moss, adaptive migration of cells, and similar behavior in non-biological systems.
(Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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