Influence of infliximab therapy on bone healing post-dental extraction in rats.

Autor: Ferreira-Junior AEC; Departament of Dental Clinic - Federal University of Ceara, Fortaleza, Ceará, Brazil., Barros-Silva PG; Departament of Dental Clinic - Federal University of Ceara, Fortaleza, Ceará, Brazil., Oliveira CC; Departament of Dental Clinic - Federal University of Ceara, Fortaleza, Ceará, Brazil., Lima Verde MEQ; Departament of Dental Clinic - Federal University of Ceara, Fortaleza, Ceará, Brazil., Sousa FB; Departament of Dental Clinic - Federal University of Ceara, Fortaleza, Ceará, Brazil., Mota MRL; Departament of Dental Clinic - Federal University of Ceara, Fortaleza, Ceará, Brazil. Electronic address: mariolmota@yahoo.com.br., Lima-Júnior RCP; Department of Physiology and Pharmacology - Federal University of Ceara, Fortaleza, Ceará, Brazil., Alves APNN; Departament of Dental Clinic - Federal University of Ceara, Fortaleza, Ceará, Brazil.
Jazyk: angličtina
Zdroj: Archives of oral biology [Arch Oral Biol] 2020 Apr; Vol. 112, pp. 104680. Date of Electronic Publication: 2020 Feb 07.
DOI: 10.1016/j.archoralbio.2020.104680
Abstrakt: Objective: TNF-α, which acts directly on osteoclastogenesis, may modify bone turnover. Thus, the objective of this study was to evaluate the influence of infliximab on extraction socket healing.
Material and Methods: Eighty-four Wistar rats were randomized into two groups (infliximab EV 5 mg / kg or saline EV 1 ml / kg) and submitted to lower first molar extraction protocol. The animals were sacrificed 1, 3, 7, 14, 21 and 28 days after surgery. The jaws were subjected to radiographic, histomorphometric, histochemical (picrosirius red) and immunohistochemical (TNF-α, RANKL and OPG) analysis.
Results: No differences were observed between the groups in surgical difficulty parameters: mass of teeth, number of root fractures and surgical time. Lower area filling with bone as well as increased amounts of remaining cicatricial tissue were observed in the infliximab group at 14 days (p < 0.001). Lower scores for polymorphonuclear neutrophils were seen at 3 (p < 0.01) and 7 days (p < 0.001), lower mononuclear counts at 7 days (p < 0.01) and lower osteoclast counts at 7 and 14 days (p < 0.01 and p < 0.001, respectively). Additionally, reduced TNF-α, RANKL and OPG immunoreactivity were observed, especially at 7 days (p < 0.05).
Conclusion: TNF-α inhibitor may alter the bone repair capacity after tooth extraction, especially in the initial repair periods, by lower expression of TNF α, RANKL and OPG. Thus, additional caution may be needed in patients who use this class of medication after dental extraction.
Competing Interests: Declaration of Competing Interest The authors have no conflicts of interest.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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