| Autor: |
Khalafalla AI; Veterinary Laboratories Division, Abu Dhabi Agriculture and Food Safety Authority (ADAFSA), Abu Dhabi, United Arab Emirates., Rashid J; Al Wathba Stables, Abu Dhabi, United Arab Emirates., Khan RA; Veterinary Laboratories Division, Abu Dhabi Agriculture and Food Safety Authority (ADAFSA), Abu Dhabi, United Arab Emirates., Alamin KM; Al Khatem Veterinary Clinic, Abu Dhabi Agriculture and Food Safety Authority (ADAFSA), Abu Dhabi, United Arab Emirates., Benkhelil A; Zoetis, Lyon, France., De Massis F; OIE Reference Laboratory for Brucellosis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Teramo, Italy., Calistri P; OIE Reference Laboratory for Brucellosis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Teramo, Italy., Giovannini A; OIE Reference Laboratory for Brucellosis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Teramo, Italy., Khan IA; Advanced Scientific Group LLC, Abu Dhabi, United Arab Emirates., Al Hosani MA; Veterinary Laboratories Division, Abu Dhabi Agriculture and Food Safety Authority (ADAFSA), Abu Dhabi, United Arab Emirates., Al Muhairi SS; Veterinary Laboratories Division, Abu Dhabi Agriculture and Food Safety Authority (ADAFSA), Abu Dhabi, United Arab Emirates. |
| Abstrakt: |
This study was conducted to evaluate the use of Brucellergene skin test (BST) for the diagnosis of Brucellosis in camels ( Camelus dromedarius ) in comparison with Rose Bengal test (RBT) and competitive enzyme-linked immunosorbent assay (c-ELISA). A total of 68 apparently healthy adult dromedary camels of either gender from three different geographical locations of Abu Dhabi Emirate, United Arab Emirates (UAE), were included in the study. The skin test was applied on two shaved areas at the middle of the neck: one for the test and the other area was injected with normal saline as a control. Reading was done 72 h postinjection. Results were subjected to Bayesian analysis to assess the test performances in camels. The model estimated the following sensitivity and specificity median values: BST: Se = 70.72%, Sp = 98.82%; RBT: Se = 93.27%, Sp = 97.79%; and c-ELISA: Se = 94.78%, Sp = 98.48%. As the BST investigated in this study proved to be a highly specific test, we propose using it as a confirmatory test in camels particularly when the serological tests give doubtful results on individual animals. |
