Regenerating islet-derived protein (Reg)3β plays a crucial role in attenuation of ileitis and colitis in mice.

Autor:	Shindo R; Department of Biochemistry, Toho University Graduate School Medicine, 5-21-16 Omori-Nishi, Ota-ku, Tokyo, 143-8540, Japan., Katagiri T; Department of Biochemistry, Toho University Graduate School Medicine, 5-21-16 Omori-Nishi, Ota-ku, Tokyo, 143-8540, Japan.; Division of Rheumatology, Department of Internal Medicine, Ohashi Medical Center, 2-22-36 Ohashi, Meguro-ku, Tokyo, 153-8515, Japan., Komazawa-Sakon S; Department of Biochemistry, Toho University Graduate School Medicine, 5-21-16 Omori-Nishi, Ota-ku, Tokyo, 143-8540, Japan., Ohmuraya M; Department of Genetics, Hyogo College of Medicine, Nishinomiya, Hyogo, 663-8501, Japan., Takeda W; Laboratory of Molecular Biology and Immunology, Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, 6-3-1 Niijuku, Katsushika-ku, Tokyo, 125-8585, Japan., Nakagawa Y; Center for Animal Resources and Development, Kumamoto University, 2-2-1 Honjo, Chuo-ku, Kumamoto, 860-0811, Japan., Nakagata N; Center for Animal Resources and Development, Kumamoto University, 2-2-1 Honjo, Chuo-ku, Kumamoto, 860-0811, Japan., Sakuma T; Division of Integrated Sciences for Life, Graduate School of Integrated Sciences for Life, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima, Hiroshima, 739-8526, Japan., Yamamoto T; Division of Integrated Sciences for Life, Graduate School of Integrated Sciences for Life, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima, Hiroshima, 739-8526, Japan., Nishiyama C; Laboratory of Molecular Biology and Immunology, Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, 6-3-1 Niijuku, Katsushika-ku, Tokyo, 125-8585, Japan., Nishina T; Department of Biochemistry, Toho University Graduate School Medicine, 5-21-16 Omori-Nishi, Ota-ku, Tokyo, 143-8540, Japan., Yamazaki S; Department of Biochemistry, Toho University Graduate School Medicine, 5-21-16 Omori-Nishi, Ota-ku, Tokyo, 143-8540, Japan., Kameda H; Division of Rheumatology, Department of Internal Medicine, Ohashi Medical Center, 2-22-36 Ohashi, Meguro-ku, Tokyo, 153-8515, Japan., Nakano H; Department of Biochemistry, Toho University Graduate School Medicine, 5-21-16 Omori-Nishi, Ota-ku, Tokyo, 143-8540, Japan.; Host Defense Research Center, Toho University School of Medicine, 5-21-16 Omori-Nishi, Ota-ku, Tokyo, 143-8540, Japan.
Jazyk:	angličtina
Zdroj:	Biochemistry and biophysics reports [Biochem Biophys Rep] 2020 Feb 07; Vol. 21, pp. 100738. Date of Electronic Publication: 2020 Feb 07 (Print Publication: 2020).
DOI:	10.1016/j.bbrep.2020.100738
Abstrakt:	Regenerating islet-derived protein (Reg)3β belongs to a member of the Reg family of proteins and has pleiotropic functions, including antimicrobial activity and tissue repair. However, whether Reg3β plays a protective role in the development of colitis and ileitis has not been fully investigated. We generated transgenic mice expressing a short form of cellular FLICE-inhibitory protein (cFLIPs) that promotes necroptosis, a regulated form of cell death. cFLIPs transgenic ( CFLARs Tg) mice develop severe ileitis in utero. Although Reg3β is undetectable in the small intestine of wild-type embryos, its expression is aberrantly elevated in the small intestine of CFLARs Tg embryos. To test whether elevated Reg3β attenuates or exacerbates ileitis in CFLARs Tg mice, we generated a Reg3b -/- strain. Reg3b -/- mice grew to adulthood without apparent abnormalities. Deletion of Reg3b in CFLARs Tg mice exacerbated the embryonic lethality of CFLARs Tg mice. Dextran sulfate sodium-induced colitis, characterized by body weight loss and infiltration of neutrophils, was exacerbated in Reg3b -/- compared to wild-type mice. Moreover, the expression of Interleukin 6 , an inflammatory cytokine and Chitinase-like 3 , a marker for tissue repair macrophages was elevated in the colon of Reg3b -/- mice compared to wild-type mice after DSS treatment. Together, these results suggest that attenuation of colitis and ileitis is a result of Reg3β's real function. Competing Interests: The authors declare that no competing interests exist. (© 2020 The Authors.)
Databáze:	MEDLINE
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