Nucleic acid-cleaving catalytic DNA for sensing and therapeutics.

Autor: Zimmermann AC; Department of Chemistry and Biochemistry, 8051, Regents Drive, University of Maryland, College Park, MD, USA., White IM; Fischell Department of Bioengineering, 8278, Paint Branch Drive, University of Maryland, College Park, MD, USA. Electronic address: ianwhite@umd.edu., Kahn JD; Department of Chemistry and Biochemistry, 8051, Regents Drive, University of Maryland, College Park, MD, USA. Electronic address: jdkahn@umd.edu.
Jazyk: angličtina
Zdroj: Talanta [Talanta] 2020 May 01; Vol. 211, pp. 120709. Date of Electronic Publication: 2020 Jan 03.
DOI: 10.1016/j.talanta.2019.120709
Abstrakt: DNAzymes with nucleic acid-cleaving catalytic activity are increasing in versatility through concerted efforts to discover new sequences with unique functions, and they are generating excitement in the sensing community as cheap, stable, amplifiable detection elements. This review provides a comprehensive list and detailed descriptions of the DNAzymes identified to date, classified by their associated small molecule or ion needed for catalysis; of note, this classification clarifies conserved regions of various DNAzymes that are not obvious in the literature. Furthermore, we detail the breadth of functionality of these DNA sequences as well as the range of reaction conditions under which they are useful. In addition, the utility of the DNAzymes in a variety of sensing and therapeutic applications is presented, detailing both their advantages and disadvantages.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: