Surface-Dependent Osteoblasts Response to TiO 2 Nanotubes of Different Crystallinity.

Autor: Khrunyk YY; Ural Federal University, Mira Str. 19, 620002 Yekaterinburg, Russia.; Institute of High-Temperature Electrochemistry of the Ural Branch of the Russian Academy of Sciences, Akademicheskaya Str. 20, 620990 Yekaterinburg, Russia., Belikov SV; Ural Federal University, Mira Str. 19, 620002 Yekaterinburg, Russia.; M.N. Mikheev Institute of Metal Physics of the Ural Branch of the Russian Academy of Sciences, Sofia Kovalevskaya Str. 18, 620219 Yekaterinburg, Russia., Tsurkan MV; Leibniz Institute of Polymer Research Dresden, 01069 Dresden, Germany.; Max Bergmann Center of Biomaterials Dresden, Hohe Str. 6, 01069 Dresden, Germany., Vyalykh IV; Yekaterinburg Research Institute of Viral Infections, Rospotrebnadzor, Letnyaya Str. 23, 620030 Yekaterinburg, Russia., Markaryan AY; Yekaterinburg Research Institute of Viral Infections, Rospotrebnadzor, Letnyaya Str. 23, 620030 Yekaterinburg, Russia., Karabanalov MS; Ural Federal University, Mira Str. 19, 620002 Yekaterinburg, Russia., Popov AA; Ural Federal University, Mira Str. 19, 620002 Yekaterinburg, Russia., Wysokowski M; Institute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, Berdychowo 4, 60965 Poznan, Poland.
Jazyk: angličtina
Zdroj: Nanomaterials (Basel, Switzerland) [Nanomaterials (Basel)] 2020 Feb 13; Vol. 10 (2). Date of Electronic Publication: 2020 Feb 13.
DOI: 10.3390/nano10020320
Abstrakt: One of the major challenges of implantology is to design nanoscale modifications of titanium implant surfaces inducing osseointegration. The aim of this study was to investigate the behavior of rat osteoblasts cultured on anodized TiO 2 nanotubes of different crystallinity (amorphous and anatase phase) up to 24 days. TiO 2 nanotubes were fabricated on VT1-0 titanium foil via a two-step anodization at 20 V using NH 4 F as an electrolyte. Anatase-phase samples were prepared by heat treatment at 500 °C for 1 h. VT1-0 samples with flat surfaces were used as controls. Primary rat osteoblasts were seeded over experimental surfaces for several incubation times. Scanning electron microscopy (SEM) was used to analyze tested surfaces and cell morphology. Cell adhesion and proliferation were investigated by cell counting. Osteogenic differentiation of cells was evaluated by qPCR of runt-related transcription factor 2 (RUNX2), osteopontin (OPN), integrin binding sialoprotein (IBSP), alkaline phosphatase (ALP) and osteocalcin (OCN). Cell adhesion and proliferation, cell morphology and the expression of osteogenic markers were affected by TiO 2 nanotube layered substrates of amorphous and anatase crystallinity. In comparison with flat titanium, along with increased cell adhesion and cell growth a large portion of osteoblasts grown on the both nanostructured surfaces exhibited an osteocyte-like morphology as early as 48 h of culture. Moreover, the expression of all tested osteogenic markers in cells cultured on amorphous and anatase TiO 2 nanotubes was upregulated at least at one of the analyzed time points. To summarize, we demonstrated that amorphous and anodized TiO 2 layered substrates are highly biocompatible with rat osteoblasts and that the surface modification with about 1500 nm length nanotubes of 35 ± 4 (amorphous phase) and 41 ± 8 nm (anatase phase) in diameter is sufficient to induce their osteogenic differentiation. Such results are significant to the engineering of coating strategies for orthopedic implants aimed to establish a more efficient bone to implant contact and enhance bone repair.
Competing Interests: The authors declare no conflict of interest.
Databáze: MEDLINE
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