Tissue transglutaminase_variant 2-transduced mesenchymal stem cells and their chondrogenic potential.

Autor: Calikoglu Koyuncu AC; Department of Genetics and Bioengineering/Faculty of Engineering, Yeditepe University, İstanbul, Turkey., Nayman AH; Department of Genetics and Bioengineering/Faculty of Engineering, Yeditepe University, İstanbul, Turkey., Telci D; Department of Genetics and Bioengineering/Faculty of Engineering, Yeditepe University, İstanbul, Turkey., Torun Kose G; Department of Genetics and Bioengineering/Faculty of Engineering, Yeditepe University, İstanbul, Turkey.
Jazyk: angličtina
Zdroj: Biotechnology and bioengineering [Biotechnol Bioeng] 2020 Jun; Vol. 117 (6), pp. 1839-1852. Date of Electronic Publication: 2020 Mar 01.
DOI: 10.1002/bit.27311
Abstrakt: As cartilage is incapable of self-healing upon severe degeneration because of the lack of blood vessels, cartilage tissue engineering is gaining importance in the treatment of cartilage defects. This study was designed to improve cartilage tissue regeneration by expressing tissue transglutaminase variant 2 (TGM2_v2) in mesenchymal stem cells (MSC) derived from bone marrow of rats. For this purpose, rat MSCs transduced with TGM2_v2 were grown and differentiated on three-dimensional polybutylene succinate (PBSu) and poly-l-lactide (PLLA) blend scaffolds. The transduced cells could not only successfully express the short form transglutaminase-2, but also deposited the protein onto the scaffolds. In addition, they could spontaneously produce cartilage-specific proteins without any chondrogenic induction, suggesting that TGM2_v2 expression provided the cells the ability of chondrogenic differentiation. PBSu:PLLA scaffolds loaded with TGM2_v2 expressing MSCs could be used in repair of articular cartilage defects.
(© 2020 Wiley Periodicals, Inc.)
Databáze: MEDLINE
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