Implementation of ezTrack open-source pipeline for quantifying rat locomotor behavior: Comparison to commercially available software.

Autor: McElroy DL; Department of Anatomy, Physiology, and Pharmacology, University of Saskatchewan, Saskatoon, SK, Canada., Roebuck AJ; Department of Anatomy, Physiology, and Pharmacology, University of Saskatchewan, Saskatoon, SK, Canada., Onofrychuk TJ; Department of Anatomy, Physiology, and Pharmacology, University of Saskatchewan, Saskatoon, SK, Canada., Sandini TM; Department of Anatomy, Physiology, and Pharmacology, University of Saskatchewan, Saskatoon, SK, Canada., Greba Q; Department of Anatomy, Physiology, and Pharmacology, University of Saskatchewan, Saskatoon, SK, Canada., Howland JG; Department of Anatomy, Physiology, and Pharmacology, University of Saskatchewan, Saskatoon, SK, Canada. Electronic address: john.howland@usask.ca.
Jazyk: angličtina
Zdroj: Neuroscience letters [Neurosci Lett] 2020 Apr 01; Vol. 723, pp. 134839. Date of Electronic Publication: 2020 Feb 14.
DOI: 10.1016/j.neulet.2020.134839
Abstrakt: Animal tracking software is an important tool to record and analyze locomotor activity during behavioral assays that provides considerable advantages over traditional manual scoring approaches (e.g., counting line crosses on a grid overlay or using a stopwatch to score time spent in regions of interest). Although several options are available to researchers, tracking software is often costly or requires advanced technical knowledge to operate efficiently. In this study, a free open-source behavioral tracking pipeline called ezTrack was compared to commercially available software for assessing rat locomotor behavior and time spent in regions of interest during elevated plus maze (EPM) and open field test (OFT) assays. ezTrack produced nearly identical results to the commercial software. Overall, these results suggest that ezTrack is a cost-effective approach to quantify some aspects of behavior in these tasks.
Competing Interests: Declaration of Competing Interest No conflicts of interests are declared by the authors.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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