Measurement of 1α hydroxycorticosterone in the Japanese banded houndshark, Triakis scyllium, following exposure to a series of stressors.

Autor: Iki A; Laboratory of Physiology, Atmosphere and Ocean Research Institute, University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8564, Japan., Anderson WG; Laboratory of Physiology, Atmosphere and Ocean Research Institute, University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8564, Japan; Department of Biological Sciences, University of Manitoba, 50 Sifton Road, Winnipeg, MB R3T 2N2, Canada., Deck CA; Department of Biological Sciences, North Carolina State University, 3510 Thomas Hall Campus Box 7614 Raleigh, NC 27695, USA., Ogihara MH; Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8562, Japan., Ikeba K; Laboratory of Physiology, Atmosphere and Ocean Research Institute, University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8564, Japan., Kataoka H; Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8562, Japan., Hyodo S; Laboratory of Physiology, Atmosphere and Ocean Research Institute, University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8564, Japan. Electronic address: hyodo@aori.u-tokyo.ac.jp.
Jazyk: angličtina
Zdroj: General and comparative endocrinology [Gen Comp Endocrinol] 2020 Jun 01; Vol. 292, pp. 113440. Date of Electronic Publication: 2020 Feb 14.
DOI: 10.1016/j.ygcen.2020.113440
Abstrakt: An endocrine glucocorticoid response following exposure to a stressor has been well described for many vertebrates. However, despite demonstration of secondary stress responses in a number of elasmobranchs, our understanding of the endocrine control of these responses is lacking. This is largely due to the unusual structure of the dominant corticosteroid in elasmobranch fish, 1α-hydroxycorticosterone (1α-OH-B). Here we describe plasma extraction and HPLC separation procedures that allowed for the measurement of 1α-OH-B and corticosterone from plasma samples in the cannulated, conscious free-swimming Japanese banded houndshark, Triakis scyllium. While patterns of concentration in the plasma for 1α-OH-B and corticosterone were found to be similar in all experiments conducted, circulating levels of 1α-OH-B were consistently 100-fold greater than circulating levels of corticosterone. Immediately following cannulation surgery, circulating levels of 1α-OH-B increased 7-fold compared to pre-surgery levels, while the levels were 11-fold higher than pre-stress levels 30 min post a repeated handling/air-exposure stress. A three week period of fasting resulted in a 22-fold increase in circulating levels of 1α-OH-B in the banded houndshark. This is the first report of direct measurement of changes in circulating levels of the primary corticosteroid in elasmobranch fish, 1α-OH-B, following exposure to a stressor such as handling/air-exposure. Data indicate the steroid may respond similarly to the classic glucocorticoid response, such as cortisol in teleosts.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2020 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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