Distinct microbial and immune niches of the human colon.

Autor: James KR; Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, UK. kj7@sanger.ac.uk., Gomes T; Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, UK., Elmentaite R; Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, UK., Kumar N; Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, UK., Gulliver EL; Department of Molecular and Translational Sciences, Monash University, Clayton, Victoria, Australia., King HW; Centre for Immunobiology, Blizard Institute, Queen Mary University of London, London, UK., Stares MD; Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, UK., Bareham BR; Department of Surgery, University of Cambridge and NIHR Cambridge Biomedical Research Centre, Cambridge, UK., Ferdinand JR; Molecular Immunity Unit, Department of Medicine, University of Cambridge, MRC Laboratory of Molecular Biology, Cambridge, UK., Petrova VN; Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, UK., Polański K; Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, UK., Forster SC; Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, UK.; Department of Molecular and Translational Sciences, Monash University, Clayton, Victoria, Australia.; Centre for Innate Immunity and Infectious Diseases, Hudson Institute of Medical Research, Clayton, Victoria, Australia., Jarvis LB; Department of Haematology, Clifford Allbutt Building, Cambridge, UK., Suchanek O; Molecular Immunity Unit, Department of Medicine, University of Cambridge, MRC Laboratory of Molecular Biology, Cambridge, UK., Howlett S; Department of Haematology, Clifford Allbutt Building, Cambridge, UK., James LK; Centre for Immunobiology, Blizard Institute, Queen Mary University of London, London, UK., Jones JL; Department of Haematology, Clifford Allbutt Building, Cambridge, UK.; Cambridge University Hospitals NHS Foundation Trust, Cambridge, UK., Meyer KB; Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, UK., Clatworthy MR; Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, UK.; Molecular Immunity Unit, Department of Medicine, University of Cambridge, MRC Laboratory of Molecular Biology, Cambridge, UK., Saeb-Parsy K; Department of Surgery, University of Cambridge and NIHR Cambridge Biomedical Research Centre, Cambridge, UK., Lawley TD; Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, UK., Teichmann SA; Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, UK. st9@sanger.ac.uk.; Theory of Condensed Matter, Cavendish Laboratory, Department of Physics, University of Cambridge, Cambridge, UK. st9@sanger.ac.uk.; European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton, UK. st9@sanger.ac.uk.
Jazyk: angličtina
Zdroj: Nature immunology [Nat Immunol] 2020 Mar; Vol. 21 (3), pp. 343-353. Date of Electronic Publication: 2020 Feb 17.
DOI: 10.1038/s41590-020-0602-z
Abstrakt: Gastrointestinal microbiota and immune cells interact closely and display regional specificity; however, little is known about how these communities differ with location. Here, we simultaneously assess microbiota and single immune cells across the healthy, adult human colon, with paired characterization of immune cells in the mesenteric lymph nodes, to delineate colonic immune niches at steady state. We describe distinct helper T cell activation and migration profiles along the colon and characterize the transcriptional adaptation trajectory of regulatory T cells between lymphoid tissue and colon. Finally, we show increasing B cell accumulation, clonal expansion and mutational frequency from the cecum to the sigmoid colon and link this to the increasing number of reactive bacterial species.
Databáze: MEDLINE
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