| Autor: |
Guéniot L; Institut Pasteur, Experimental Neuropathology Unit, Paris, France.; Direction Générale de l'Armement, Ministère des Armées, Paris, France.; Paris Descartes University, ED Bio-SPC, Sorbonne Paris Cité, Paris, France., Lepere V; Institut Pasteur, Experimental Neuropathology Unit, Paris, France.; Multidisciplinary Intensive Care Unit, Department of Anesthesiology and Critical Care, La Pitié-Salpêtrière Hospital, Assistance Publique-Hôpitaux de Paris, Sorbonne Université, Paris, France.; Polyvalent Surgical Resuscitation, La Pitié-Salpêtrière Hospital, Assistance Publique-Hôpitaux de Paris, Sorbonne University, Paris, France., De Medeiros GF; Institut Pasteur, Experimental Neuropathology Unit, Paris, France., Danckaert A; Institut Pasteur, UtechS Photonic BioImaging (Imagopole), C2RT, Paris, France., Flamant P; Institut Pasteur, Experimental Neuropathology Unit, Paris, France., Le Dudal M; Institut Pasteur, Experimental Neuropathology Unit, Paris, France., Langeron O; Institut Pasteur, Experimental Neuropathology Unit, Paris, France.; Department of Anesthesia and Critical Care, Hôpitaux Universitaires Henri Mondor-Créteil-Assistance Publique Hôpitaux de Paris, Paris-Est Créteil University, Créteil, France., Goossens PL; Institut Pasteur, Experimental Neuropathology Unit, Paris, France., Chrétien F; Institut Pasteur, Experimental Neuropathology Unit, Paris, France.; Paris Descartes University, ED Bio-SPC, Sorbonne Paris Cité, Paris, France.; Service de Neuropathologie, Centre Hospitalier Sainte Anne, GHU Paris Psychiatrie Neuroscience, Paris, France., Jouvion G; Institut Pasteur, Experimental Neuropathology Unit, Paris, France. gregory.jouvion@pasteur.fr.; Sorbonne Université, INSERM, Pathophysiology of pediatric genetic diseases, Assistance Publique-Hôpitaux de Paris, Hôpital Armand-Trousseau, UF Génétique moléculaire, Paris, France. gregory.jouvion@pasteur.fr. |
| Abstrakt: |
Postoperative cognitive dysfunction (POCD) is a major complication affecting patients of any age undergoing surgery. This syndrome impacts everyday life up to months after hospital discharge, and its pathophysiology still remains unclear. Translational research focusing on POCD is based on a wide variety of rodent models, such as the murine tibial fracture, whose severity can limit mouse locomotion and proper behavioral assessment. Besides, influence of skeletal muscle injury, a lesion encountered in a wide range of surgeries, has not been explored in POCD occurrence. We propose a physical model of muscle injury in CX3CR1 GFP/+ mice (displaying green fluorescent microglial cells) to study POCD, with morphological, behavioral and molecular approaches. We highlighted: alteration of short- and long-term memory after muscle regeneration, wide microglial reactivity in the brain, including hippocampus area, 24 hours after muscle injury, and an alteration of central brain derived neurotrophic factor (BDNF) and nerve growth factor (NGF) balance, 28 days after muscle injury. Our results suggest for the first time that muscle injury can have early as well as late impacts on the brain. Our CX3CR1 GFP/+ model can also facilitate microglial investigation, more specifically their pivotal role in neuroinflammation and synaptic plasticity, in the pathophysiology of POCD. |
